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master's thesis
Kemijski sastav i biološki učinak ekstrakta cvijeta maslačka (Taraxacum officinale)
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:093851
Širić, Ariana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Širić, A. (2016). Kemijski sastav i biološki učinak ekstrakta cvijeta maslačka (Taraxacum officinale) (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:093851

Širić, Ariana. "Kemijski sastav i biološki učinak ekstrakta cvijeta maslačka (Taraxacum officinale)." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:093851

Širić, Ariana. "Kemijski sastav i biološki učinak ekstrakta cvijeta maslačka (Taraxacum officinale)." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:093851

Širić, A. (2016). 'Kemijski sastav i biološki učinak ekstrakta cvijeta maslačka (Taraxacum officinale)', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:093851

Širić A. Kemijski sastav i biološki učinak ekstrakta cvijeta maslačka (Taraxacum officinale) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 December 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:093851

A. Širić, "Kemijski sastav i biološki učinak ekstrakta cvijeta maslačka (Taraxacum officinale)", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:093851

Metadata
TitleKemijski sastav i biološki učinak ekstrakta cvijeta maslačka (Taraxacum officinale)
Title (english)Chemical composition and biological effect of the dandelion flower extract (Taraxacum officinale)
AuthorAriana Širić
MentorKsenija Durgo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2016-01-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Ljekovite biljke sadrže više različitih kemijskih tvari koje djeluju povoljno na zdravlje, pojedinačno, kumulativno ili u sinergiji s drugim tvarima. U ovom radu je ispitan kemijski i bioaktivni profil cvijeta maslačka, određen je biološki učinak ekstrakta cvijeta maslačka na Cal27, Cal27-2B1 i T24 stanice te je istražen citotoksični učinak na različite vrste laktobacila. Određen je udio ukupnih polifenolnih spojeva i pojedinih skupina te pigmentnih spojeva i topljivih polisaharida pri čemu su hidroksicimetne kiseline i karotenoidi (β-karoten) zastupljeni u visokom udjelu. Utvrđeno je da ekstrakt cvijeta maslačka ima približno isti antioksidacijski kapacitet bez obzira na primijenjenu metodu određivanja. Ekstrakt cvijeta maslačka pokazuje citotoksični učinak kod staničnih linija raka jezika, pri čemu je epitel jezika s eksprimiranim integrinom najosjetljiviji. Ekstrakt maslačka djeluje slabo prooksidativno, a indukcija slobodnih radikala ne utječe na promjenu preživljenja staničnih linija. Ekstrakt cvijeta maslačka nije toksičan za odabrane sojeve laktobacila te njegovom upotrebom neće doći do narušavanja ravnoteže mikroflore u sisavaca.
Abstract (english)
Medicinal plants contain different chemical compounds that may act individually, additively, or in synergy to improve health. In this study the chemical and bioactive profile of the dandelion flower extract was evaluated as well as its biological effect on the Cal27, Cal27-2B1 and T24 cell lines. The plant extract was evaluated for its total phenolic content, specific phenolic compounds, pigments and soluble polysaccharides. It contains a high level of hydroxycinnamic acids and carotenoids (β-carotene). The dandelion flower extract had similar antioxidant capacities despite the three different methods used. The extract exhibited citotoxic activity against the tongue carcinoma cell lines, wherein the tongue epithelium with expressed integrin was the most sensitive one. The extract showed low prooxidative effect and the free radical induction had no influence on cell viability. The antimicrobial activity against selected lactic acid bacteria was observed wherein the extract exhibited no inhibitory effect which means that the consumption of dandelion flower extract will not compromise the microbial balance in mammals.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:093851
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-27 08:58:52