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master's thesis
Priprema i biološka aktivnost ekstrakata nusproizvoda vinske industrije pripravljenih pomoću niskotemperaturnih eutektičkih otapala
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:053280
Dvorneković, Iva
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Dvorneković, I. (2021). Priprema i biološka aktivnost ekstrakata nusproizvoda vinske industrije pripravljenih pomoću niskotemperaturnih eutektičkih otapala (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:053280

Dvorneković, Iva. "Priprema i biološka aktivnost ekstrakata nusproizvoda vinske industrije pripravljenih pomoću niskotemperaturnih eutektičkih otapala." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:053280

Dvorneković, Iva. "Priprema i biološka aktivnost ekstrakata nusproizvoda vinske industrije pripravljenih pomoću niskotemperaturnih eutektičkih otapala." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:053280

Dvorneković, I. (2021). 'Priprema i biološka aktivnost ekstrakata nusproizvoda vinske industrije pripravljenih pomoću niskotemperaturnih eutektičkih otapala', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 05 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:053280

Dvorneković I. Priprema i biološka aktivnost ekstrakata nusproizvoda vinske industrije pripravljenih pomoću niskotemperaturnih eutektičkih otapala [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 August 05] Available at: https://urn.nsk.hr/urn:nbn:hr:159:053280

I. Dvorneković, "Priprema i biološka aktivnost ekstrakata nusproizvoda vinske industrije pripravljenih pomoću niskotemperaturnih eutektičkih otapala", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:053280

Metadata
TitlePriprema i biološka aktivnost ekstrakata nusproizvoda vinske industrije pripravljenih pomoću niskotemperaturnih eutektičkih otapala
Title (english)Preparation and biological activity of wine industry by-products extracts prepared using deep eutectic solvents
AuthorIva Dvorneković
MentorIvana Radojčić Redovniković (mentor)
Committee memberKristina Radošević (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2021-11-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Za zelenu ekstrakciju biološki aktivnih spojeva posljednjih se godina često upotrebljavaju prirodna niskotemperaturna eutektička otapala (NADES), kao potencijalna zamjena konvencionalnim otapalima. Nakon proizvodnje vina zaostaju nusprodukti, pokožica i sjemenke grožđa, bogati polifenolima koji se mogu primijeniti u kozmetičkoj industriji. U ovom radu su pripremljeni ekstrakti pokožice i sjemenki grožđa u NADES-ima prethodno dizajniranima pomoću COSMOtherm programa i etanolu. Ukupan sadržaj polifenola u ekstraktima određen je Folin-Ciocalteu reagensom, određeni polifenoli identificirani su i kvatificirani tekućinskom kromatografijom visoke djelotvornosti, dok je antioksidacijski učinak ekstrakata određen ORAC metodom. Biološka aktivnost pripravljenih ekstrakata ispitana je in vitro na HaCaT staničnoj liniji MTS metodom, a antioksidacijski potencijal u stanicama DCFA-DA metodom. Najbolji učinak ekstrakcije postignut je eutektičkim otapalom betain : glukoza za pokožicu, a betain : etilen-glikolom za sjemenke. HPLC analizom određena je prisutnost epigalokatehina, katehina, epikatehina, rutin trihidrata i derivata flavan-3-ola u ekstraktima pokožice i sjemenka. NADES otapala, na temelju svih ispitanih parametara, mogu uspješno zamjeniti etanol kao konvencionalno otapalo u procesu ekstrakcije. S obzirom na uoče
Abstract (english)
In recent years, natural deep eutectic solvents (NADES) have often been used for extraction of biologically active compounds, as a potential replacement for conventional solvents. Grape skin and seeds are wine production by-products rich in polyphenols that can be used in cosmetic industry. In this study, grape skin and seeds extracts were prepared in NADES previously selected by COSMOtherm and ethanol. Extracts total polyphenols content was determined with Folin-Ciocalteu reagent and certain polyphenols were identified and quantified by high-performance liquid chromatography, while antioxidant effect by the ORAC method. Biological activity of prepared extracts was evaluated in vitro on the HaCaT Cell line using the MTS and antioxidant effect in cells using the DCFH-DA assays. Betaine: glucose provided the best extraction efficiency for grape skin, betaine : ethylene glycol for grape seeds. The presence of epigallocatechin, catechin, epicatechin, rutin trihydrate and derivatives flavan-3-ols in grape skin and seeds was also shown. Based on all tested parameters, NADES can successfully replace ethanol as conventional solvent in extraction processes. Given the observed proliferation of HaCaT cells, the tested extracts have the potential for application in the cosmetics industry.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:053280
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2021-12-07 14:34:59