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master's thesis
Primjena niskotemperaturnih eutektičkih otapala za poboljšanje formulacije lijeka s derivatima ciprofloksacina kao aktivnim farmaceutskim sastojkom
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:681844
Belavić, Viktorija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Belavić, V. (2021). Primjena niskotemperaturnih eutektičkih otapala za poboljšanje formulacije lijeka s derivatima ciprofloksacina kao aktivnim farmaceutskim sastojkom (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:681844

Belavić, Viktorija. "Primjena niskotemperaturnih eutektičkih otapala za poboljšanje formulacije lijeka s derivatima ciprofloksacina kao aktivnim farmaceutskim sastojkom." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:681844

Belavić, Viktorija. "Primjena niskotemperaturnih eutektičkih otapala za poboljšanje formulacije lijeka s derivatima ciprofloksacina kao aktivnim farmaceutskim sastojkom." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:681844

Belavić, V. (2021). 'Primjena niskotemperaturnih eutektičkih otapala za poboljšanje formulacije lijeka s derivatima ciprofloksacina kao aktivnim farmaceutskim sastojkom', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 31 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:681844

Belavić V. Primjena niskotemperaturnih eutektičkih otapala za poboljšanje formulacije lijeka s derivatima ciprofloksacina kao aktivnim farmaceutskim sastojkom [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 December 31] Available at: https://urn.nsk.hr/urn:nbn:hr:159:681844

V. Belavić, "Primjena niskotemperaturnih eutektičkih otapala za poboljšanje formulacije lijeka s derivatima ciprofloksacina kao aktivnim farmaceutskim sastojkom", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:681844

Metadata
TitlePrimjena niskotemperaturnih eutektičkih otapala za poboljšanje formulacije lijeka s derivatima ciprofloksacina kao aktivnim farmaceutskim sastojkom
Title (english)Application of deep eutectic solvents for improvement of drug formulation with ciprofloxacin derivatives as an active pharmaceutical ingredient
AuthorViktorija Belavić
MentorKristina Radošević (mentor)
Committee memberSenka Djaković (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2021-12-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovoga rada je poboljšanje topljivosti aktivnih farmaceutskih sastojaka (API) primjenom prirodnih niskotemperaturnih eutektičkih otapala (NADES) kao zelenih otapala te sinteza derivata ciprofloksacina zbog sve većeg problema antibiotičke rezistencije spram tog lijeka. Upotrebom softvera COSMOtherm predviđena je topljivost dva 7-(4-(supstituiran)piperazin-1-il) derivata ciprofloksacina u NADES-ima, pri čemu je najbolja topljivost predviđena u kiselim i hidrofobnim otapalima. UV/Vis spektrofotometrijom je eksperimentalno određena najbolja topljivost derivata u hidrofobnom NADES-u timol:kumarin (3:2) koja iznosi 10 mg mLˉ¹ za N-acetilirani i 5 mg mLˉ¹ za N-2-metilpentanoilni derivat. Stabilnost formulacija praćena je spektrofotometrijski tijekom vremena pri 4 °C i 25 °C te su derivati stabilni prvih desetak dana, nakon čega je došlo do pada stabilnosti. Testom propusnosti kroz umjetnu membranu (PAMPA) uočeno je poboljšanje propusnosti N-acetiliranog derivata u formulaciji s NADES-om, ali ne i za drugi derivat. Ispitana je biološka aktivnost otopina derivata ciprofloksacina na Caco-2 staničnoj liniji, pri čemu je primijećeno inhibitorno djelovanje na rast stanica.
Abstract (english)
The aim of this work is to improve the solubility of active pharmaceutical ingredients (API) by using natural deep eutectic solvents (NADES) as green solvents and the synthesis of ciprofloxacin derivatives due to the growing problem of antibiotic resistance. Using COSMOtherm software, the solubility of two 7-(4-(substituted)piperazine-1-yl) ciprofloxacin derivatives in NADESs was predicted, with the best solubility in acidic and hydrophobic solvents. Solubility was experimentally determined by UV/Vis spectrophotometry and the best solubility of derivatives was in the hydrophobic NADES thymol: coumarin (3:2) of 10 mg mLˉ¹ for N-acetyl and 5 mg mLv for N-2-methylpentanoyl derivative. The stability of the formulation was monitored spectrophotometrically over the time at 4 °C and 25 °C and the derivatives were stable for the first ten days, afterwards a decrease in stability was observed. The parallel artificial membrane permeability assay (PAMPA) was conducted and improvement of permeability N-acetyl derivative in the formulation with NADES was detected, but not for another derivative. The biological activity of solution of ciprofloxacin derivatives on the Caco-2 cell line was examined, and an inhibitory effect on cell growth was determined.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:681844
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2022-01-31 13:26:27