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master's thesis
Molekularne detekcijske probe specifične za Netrin-1 - novi biomarker za metastatski oblik raka dojke: od malih molekula do liposoma
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:840515
Lukačević, Tea
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Lukačević, T. (2021). Molekularne detekcijske probe specifične za Netrin-1 - novi biomarker za metastatski oblik raka dojke: od malih molekula do liposoma (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:840515

Lukačević, Tea. "Molekularne detekcijske probe specifične za Netrin-1 - novi biomarker za metastatski oblik raka dojke: od malih molekula do liposoma." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:840515

Lukačević, Tea. "Molekularne detekcijske probe specifične za Netrin-1 - novi biomarker za metastatski oblik raka dojke: od malih molekula do liposoma." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:840515

Lukačević, T. (2021). 'Molekularne detekcijske probe specifične za Netrin-1 - novi biomarker za metastatski oblik raka dojke: od malih molekula do liposoma', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 12 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:840515

Lukačević T. Molekularne detekcijske probe specifične za Netrin-1 - novi biomarker za metastatski oblik raka dojke: od malih molekula do liposoma [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2024 July 12] Available at: https://urn.nsk.hr/urn:nbn:hr:159:840515

T. Lukačević, "Molekularne detekcijske probe specifične za Netrin-1 - novi biomarker za metastatski oblik raka dojke: od malih molekula do liposoma", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:840515

Metadata
TitleMolekularne detekcijske probe specifične za Netrin-1 - novi biomarker za metastatski oblik raka dojke: od malih molekula do liposoma
Title (english)Targeted imaging probes for Netrin-1 - a novel metastatic breast cancer biomarker: small molecules to liposomes
AuthorTea Lukačević
MentorVladimir Mrša (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2021-09-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Despite significant development of cancer imaging and treatment over the years, the challenge of early cancer diagnosis and detection of metastasis remains. Molecular imaging probes for magnetic resonance imaging (MRI, a non-invasive clinically used imaging technique with high resolution) are well adapted to solve this problem, namely via the design of new contrast agents, which target specific tumor biomarkers. The protein Netrin-1 is an extracellular protein involved in regulation of cell migration and apoptosis. This study focuses on the evaluation of Netrin-1 as a potential biomarker in metastatic breast cancer imaging. Two types of contrast agents targeted to Netrin-1 are explored: small molecule-based probes and nanoparticles (liposomes). The designed MRI probes were examined using in vitro binding assays and measuring relaxivities to assess their potential MRI application. Herein we have shown successful selectivity of both types of probes towards Netrin-1 and significant increase in relaxivity values, especially in case of nanoparticles.
Abstract (croatian)
Unatoč značajnim naprecima u dijagnostici i liječenju raka tijekom posljednjih godina, rano otkrivanje raka i detekcija metastaza predstavlja nepremošćen izazov. Molekularne detekcijske probe za magnetsku rezonancu (MR, ne invazivna klinička dijagnostička metoda visoke rezolucije) dobri su kandidati za rješavanje ovog problema, posebice kao osnova za razvijanje novih kontrasta specifičnih za određene tumorske biomarkere. Protein Netrin-1 je izvanstanični protein koji je uključen u procese migracije stanica i apoptoze. Provedeno istraživanje usmjereno je na ispitivanje potencijala proteina Netrin-1 kao mogućeg biomarkera u dijagnostici metastatskog karcinoma dojke. Korištene su dvije vrste kontrastnih agenata specifičnih za protein Netrin-1 : male molekularne probe i nanočestice (liposomi). Razvijene MR probe su ispitane koristeći in vitro testove afinitetnog vezivanja te mjerenjem relaksivnosti kako bi se ocijenila uspješnost njihove moguće primjene u MR dijagnostici. U ovom radu prikazana su uspješno uspostavljena specifičnost obje vrste proba prema proteinu Netrin-1 te značajna povećanja u vrijednostima relaksivnosti, posebice u slučaju nanočestica.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:840515
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2022-05-26 11:28:24