prikaz prve stranice dokumenta Effect of OA689 on nicotinic acetylcholine receptors expressed on neurosecretory cells
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master's thesis
Effect of OA689 on nicotinic acetylcholine receptors expressed on neurosecretory cells
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. urn:nbn:hr:159:250573
Čupić, Ivona
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Čupić, I. (2021). Effect of OA689 on nicotinic acetylcholine receptors expressed on neurosecretory cells (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:250573

Čupić, Ivona. "Effect of OA689 on nicotinic acetylcholine receptors expressed on neurosecretory cells." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:250573

Čupić, Ivona. "Effect of OA689 on nicotinic acetylcholine receptors expressed on neurosecretory cells." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2021. https://urn.nsk.hr/urn:nbn:hr:159:250573

Čupić, I. (2021). 'Effect of OA689 on nicotinic acetylcholine receptors expressed on neurosecretory cells', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 14 March 2025, https://urn.nsk.hr/urn:nbn:hr:159:250573

Čupić I. Effect of OA689 on nicotinic acetylcholine receptors expressed on neurosecretory cells [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2021 [cited 2025 March 14] Available at: https://urn.nsk.hr/urn:nbn:hr:159:250573

I. Čupić, "Effect of OA689 on nicotinic acetylcholine receptors expressed on neurosecretory cells", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2021. Available at: https://urn.nsk.hr/urn:nbn:hr:159:250573

Metadata
TitleEffect of OA689 on nicotinic acetylcholine receptors expressed on neurosecretory cells
Title (english)Utjecaj OA689 na nikotinske acetilkolinske receptore eksprimirane na neurosekretornim stanicama
AuthorIvona Čupić
MentorVladimir Mrša (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2021-09-06, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
The aim of this study is to characterise the mode of action of the compound OA689 on dorsal unpaired median (DUM) neurons from the cockroach P. americana, using whole-cell patch-clamp technique. The compound OA689 is a ligand of nicotinic acetylcholine receptors (nAChRs) which are expressed in both vertebrate and invertebrate species. We demonstrated that OA689 does not act as a nAChR agonist, but rather as an antagonist or a negative allosteric modulator of nAChRs. The application of the compound at 8.29 μM reduces acetylcholine-induced current amplitudes by 50%. The inhibitory effect of OA689 was blocked in the presence of nicotinic acetylcholine receptor antagonists, α-bungarotoxin (α-Bgt) and methyllicaconitine (MLA), while the effect of OA689 was not blocked in the presence of mecamylamine (MEC). Our data support an action of OA689 through α-Bgt-sensitive homomeric α7 receptor. The same results were obtained when the effect of OA689 alone and in the presence of nAChR antagonists was examined on nicotine-induced currents suggesting that muscarinic receptors are not responsible for the inhibitory effect of OA689.
Abstract (croatian)
Cilj ovog istraživanja je okarakterizirati način djelovanja spoja OA689 na dorzalno neparnim medijalnim (eng. dorsal unpaired median, DUM) neuronima žohara P. americana, koristeći tehniku „whole-cell patch-clamp“. Spoj OA689 je ligand nikotinskih acetilkolinskih receptora (eng. nicotinic acetylcholine receptors, nAChRs) koji su eksprimirani i kod kralježnjaka i kod beskralježnjaka. Pokazano je da OA689 ne djeluje kao agonist nikotinskih acetilkolinskih receptora, već kao antagonist ili negativni alosterički modulator. Primjena spoja pri koncentraciji 8.29 μM smanjuje amplitude struje inducirane acetilkolinom za 50 %. Inhibitorni učinak OA689 je blokiran u prisutnosti antagonista nikotinskih acetilkolinskih receptora, α-bungarotoksina (α-Bgt) i metilikakonitina (MLA), dok učinak OA689 nije blokiran u prisutnosti mekamilamina (MEC). Rezultati dobiveni ovim istraživanjem podržavaju djelovanje OA689 putem α-Bgt-osjetljivog homomernog α7 receptora. Isti rezultati su postignuti kada je ispitivan učinak OA689 te učinak OA689 u prisutnosti antagonista nAChR na struje inducirane nikotinom, što ukazuje na to da muskarinski receptori nisu odgovorni za inhibitorni učinak OA689.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:250573
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsClosed access
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Created on2022-05-26 11:33:49