prikaz prve stranice dokumenta Ekspresijska analiza čimbenika uključenih u popravak unakrsnih veza DNA-protein
  Download
PDF 1.25 MB
master's thesis
Ekspresijska analiza čimbenika uključenih u popravak unakrsnih veza DNA-protein
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:952991
Medved, Vanna
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Medved, V. (2022). Ekspresijska analiza čimbenika uključenih u popravak unakrsnih veza DNA-protein (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:952991

Medved, Vanna. "Ekspresijska analiza čimbenika uključenih u popravak unakrsnih veza DNA-protein." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:952991

Medved, Vanna. "Ekspresijska analiza čimbenika uključenih u popravak unakrsnih veza DNA-protein." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:952991

Medved, V. (2022). 'Ekspresijska analiza čimbenika uključenih u popravak unakrsnih veza DNA-protein', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:952991

Medved V. Ekspresijska analiza čimbenika uključenih u popravak unakrsnih veza DNA-protein [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 December 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:952991

V. Medved, "Ekspresijska analiza čimbenika uključenih u popravak unakrsnih veza DNA-protein", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:952991

Metadata
TitleEkspresijska analiza čimbenika uključenih u popravak unakrsnih veza DNA-protein
Title (english)Expression analysis of factors involved in the repair of DNA-protein crosslinks
AuthorVanna Medved
MentorIgor Stuparević (mentor)
Committee memberMarta Popović (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2022-03-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
DNA je kontinuirano izložena agensima koji uzrokuju oštećenja, a integritet DNA posebno narušavaju unakrsne veze DNA i proteina (engl. DNA-protein crosslink, DPC). Navedeno oštećenje ugrožava osnovne transakcije DNA molekule, zbog čega se kod ljudi akumulacija DPC-ova povezuje sa starenjem, nastankom karcinoma i neurodegeneracijom. Proteini Acrc, Zatt i Xpa imaju ulogu u različitim mehanizmima popravka DPC-ova. Acrc vjerojatno provodi proteolizu oštećenja, Zatt vjerojatno sudjeluje u procesu hidrolize unakrsne veze, a Xpa je dio puta popravka koji je ovisan o nukleaznoj aktivnosti. U ovome radu, provedena je ekspresijska analiza gena ACRC, ZATT i XPA u tkivima domaćeg miša (Mus musculus), te usporedba ekspresijskih profila ovisno o dobi i spolu. Također, ekspresijski profili proučavanih gena uspoređeni su sa ekspresijom kod čovjeka.
Abstract (english)
DNA is constantly exposed to agents that cause damage. DNA integrity is particularly affected by DNA-protein crosslink (DPC). DPC lesions threaten basic DNA transactions, which is why in humans the accumulation of DPCs is associated with aging, cancer and neurodegeneration. The proteins Acrc, Zatt, and Xpa play roles in various mechanisms of DPC repair. Acrc likely performs DPC proteolysis, Zatt is probably involved in the process of crosslink hydrolysis, and Xpa is part of a repair pathway that is dependent on nuclease activity. In this work, expression analysis of the genes ACRC, ZATT, and XPA was performed in the tissues of the house mouse (Mus musculus) and expression profiles were compared as a function of age and sex. In addition, the expression profiles of the studied genes were compared with the expression in humans.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:952991
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2022-05-27 10:03:38