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master's thesis
Inhibicija uzročnika račje kuge, Aphanomyces astaci Schikora 1906, bakterijskim izolatima s kutikule rakova
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:758161
Vukšić, Lucija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Vukšić, L. (2022). Inhibicija uzročnika račje kuge, Aphanomyces astaci Schikora 1906, bakterijskim izolatima s kutikule rakova (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:758161

Vukšić, Lucija. "Inhibicija uzročnika račje kuge, Aphanomyces astaci Schikora 1906, bakterijskim izolatima s kutikule rakova." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:758161

Vukšić, Lucija. "Inhibicija uzročnika račje kuge, Aphanomyces astaci Schikora 1906, bakterijskim izolatima s kutikule rakova." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:758161

Vukšić, L. (2022). 'Inhibicija uzročnika račje kuge, Aphanomyces astaci Schikora 1906, bakterijskim izolatima s kutikule rakova', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:758161

Vukšić L. Inhibicija uzročnika račje kuge, Aphanomyces astaci Schikora 1906, bakterijskim izolatima s kutikule rakova [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:159:758161

L. Vukšić, "Inhibicija uzročnika račje kuge, Aphanomyces astaci Schikora 1906, bakterijskim izolatima s kutikule rakova", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:758161

Metadata
TitleInhibicija uzročnika račje kuge, Aphanomyces astaci Schikora 1906, bakterijskim izolatima s kutikule rakova
Title (english)Inhibition of crayfish plague pathogen, Aphanomyces astaci Schikora 1906, by bacterial isolates from crayfish exoskeleton
AuthorLucija Vukšić
MentorAna Bielen (mentor)
Committee memberReno Hrašćan (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2022-06-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Račja kuga, uzrokovana patogenom Aphanomyces astaci, ugrožava prirodne populacije slatkovodnih rakova te uzrokuje ekonomske gubitke u akvakulturi rakova. Cilj ovog rada bio je istražiti inhibicijski potencijal bakterijskih izolata s kutikule rakova prema rastu micelija patogena A. astaci. Analizom gena koji kodira za 16S rRNA identificirano je 145 bakterijskih izolata s kutikule signalnog, uskoškarog, riječnog, potočnog i bjelonogog raka. Neovisno o vrsti raka, najveći udio izolata bio je iz koljena Proteobacteria (66 %, uključujući najčešće rodove Pseudomonas i Aeromonas), zatim Bacteroidetes (18 %, s najčešćim rodom Flavobacterium), Firmicutes (13 %), Actinobacteria (2 %) i Cyanobacteria (1 %). Nadalje, provedeni su in vitro testovi inhibicije prema kojima su izolati razvrstani u inhibitore (13 %, najčešće iz rodova Pseudomonas i Aeromonas) i ne-inhibitore (87 %). Zaključno, rezultati ovog rada otvaraju mogućnost primjene bakterija kao ekološki prihvatljive metode kontrole patogena A. astaci u akvakulturi.
Abstract (english)
Crayfish plague, caused by the pathogen Aphanomyces astaci, threatens natural freshwater crayfish and causes economic losses in astaciculture. The aim of this study was to investigate the inhibitory potential of bacterial isolates from the crayfish exoskeleton against mycelial growth of A. astaci. A total of 145 bacterial isolates from the exoskeleton of signal crayfish, narrow-clawed crayfish, noble crayfish, stone crayfish and white-clawed crayfish were identified by 16S rRNA gene analysis. Regardless of the crayfish species, most isolates belonged to the phylum Proteobacteria (66 %, including the most common genera Pseudomonas and Aeromonas), followed by Bacteroidetes (18 %, most commonly the genus Flavobacterium), Firmicutes (13 %), Actinobacteria (2 %) and Cyanobacteria (1 %). In addition, in vitro inhibition tests were performed, according to which the isolates were classified as inhibitors (13 %, most frequently from the genera Pseudomonas and Aeromonas) or non-inhibitors (87 %). The results of this work open up the perspective of using bacteria as an environmentally acceptable method of controlling A. astaci in aquaculture.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:758161
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2022-06-29 11:51:30