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undergraduate thesis
Utjecaj litičkih polisaharidnih monooksigenaza na nanocelulozu
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:194006
Anđelini, Magdalena
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Anđelini, M. (2022). Utjecaj litičkih polisaharidnih monooksigenaza na nanocelulozu (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:194006

Anđelini, Magdalena. "Utjecaj litičkih polisaharidnih monooksigenaza na nanocelulozu." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:194006

Anđelini, Magdalena. "Utjecaj litičkih polisaharidnih monooksigenaza na nanocelulozu." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:194006

Anđelini, M. (2022). 'Utjecaj litičkih polisaharidnih monooksigenaza na nanocelulozu', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 16 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:194006

Anđelini M. Utjecaj litičkih polisaharidnih monooksigenaza na nanocelulozu [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2025 January 16] Available at: https://urn.nsk.hr/urn:nbn:hr:159:194006

M. Anđelini, "Utjecaj litičkih polisaharidnih monooksigenaza na nanocelulozu", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:194006

Metadata
TitleUtjecaj litičkih polisaharidnih monooksigenaza na nanocelulozu
Title (english)Effects of lytic polysaccharide monooxygenases on nanocellulose
AuthorMagdalena Anđelini
MentorTonči Rezić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2022-07-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Nanoceluloza je prirodan materijal dobiven iz lignoceluloznih sirovina mehaničkim, kemijskim ili enzimskim postupcima. Zbog svojih iznimnih fizikalno-kemijskih svojstava primjenjuje se u proizvodnji kompozitnih materijala i papira te u prehrambenoj, kozmetičkoj i biomedicinskoj industriji. Enzimski katalizirani procesi za dobivanje nanoceluloze imaju brojne prednosti nad kemijskim, a skupina enzima koji su privukli poseban interes su litičke polisaharidne monooksigenaze (LPMO). Ovi enzimi imaju bitnu ulogu u razgradnji lignoceluloze jer djeluju sinergistički s drugim enzimima tako što povećaju dostupnost celuloze za djelovanje celulaza. Kao rezultat njihove C1- oksidacijske aktivnosti, LPMO induciraju slabljenje celuloznih vlakana uvođenjem karboksilnih skupina čime se povećava prinos nanoceluloze. Za mjerenje aktivnosti LPMO-a razvijena je nova metoda koja se temelji na hidrocoerulignonu kao supstratu i vodikovom peroksidu kao kosupstratu, a omogućava pouzdanu detekciju LPMO-a u složenim matricama poput fermentacijskog medija.
Abstract (english)
Nanocellulose is a natural material obtained from lignocellulosic materials by mechanical, chemical or enzymatic processes. Due to its exceptional physico-chemical properties, it is used in the production of composite materials and paper, as well as in the food, cosmetics and biomedical industries. Enzymatically catalyzed processes for the production of nanocellulose have numerous advantages over chemical ones, and the group of enzymes that have attracted special interest are lytic polysaccharide monooxygenases (LPMO). These enzymes play an important role in the breakdown of lignocellulose because they act synergistically with other enzymes by increasing the availability of cellulose for cellulases. As a result of their C1-oxidizing activity, LPMOs disrupt the cellulose fibers by the introduction of carboxyl groups thus increasing the yield of nanocellulose. A new method has been developed for measuring the LPMO activity based on hydrocoerulignone as substrate and hydrogen peroxide as cosubstrate which allows reliable detection of LPMO in complex matrices like culture media.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:194006
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2022-10-05 11:38:47