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undergraduate thesis
Uporaba lančane reakcije polimerazom u sintezi fragmenta DNA za inaktivaciju kvaščevog gena YKU70
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:230236
Potkonjak, Leo
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Potkonjak, L. (2022). Uporaba lančane reakcije polimerazom u sintezi fragmenta DNA za inaktivaciju kvaščevog gena YKU70 (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:230236

Potkonjak, Leo. "Uporaba lančane reakcije polimerazom u sintezi fragmenta DNA za inaktivaciju kvaščevog gena YKU70." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:230236

Potkonjak, Leo. "Uporaba lančane reakcije polimerazom u sintezi fragmenta DNA za inaktivaciju kvaščevog gena YKU70." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:230236

Potkonjak, L. (2022). 'Uporaba lančane reakcije polimerazom u sintezi fragmenta DNA za inaktivaciju kvaščevog gena YKU70', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:230236

Potkonjak L. Uporaba lančane reakcije polimerazom u sintezi fragmenta DNA za inaktivaciju kvaščevog gena YKU70 [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 December 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:230236

L. Potkonjak, "Uporaba lančane reakcije polimerazom u sintezi fragmenta DNA za inaktivaciju kvaščevog gena YKU70", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:230236

Metadata
TitleUporaba lančane reakcije polimerazom u sintezi fragmenta DNA za inaktivaciju kvaščevog gena YKU70
Title (english)Using the Polymerase Chain Reaction to Synthesise a DNA Fragment Capable of Inactivating the YKU70 Gene
AuthorLeo Potkonjak
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2022-07-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj rada bila je sinteza disrupcijske kazete za izbacivanje gena YKU70 iz genoma kvasca Saccharomyces cerevisiae homolognom rekombinacijom. Nakon izolacije genomske DNA određenog soja tog kvasca, kazeta je sintetizirana umnažanjem sekvence yku70::KanMX4 pomoću lančane reakcije polimerazom. Uspješnost umnažanja potvrđena je restrikcijskom analizom produkta i gel elektroforezom.
Abstract (english)
The objective of this project was the synthesis of a specific DNA fragment which can be used to replace the YKU70 gene in the genome of Saccharomyces cerevisiae by means of homologous recombination. After isolating the DNA of a specific strain of this yeast, the fragment was synthesised by amplifying the yku70::KanMX4 sequence using the polymerase chain reaction. The success of the amplification was confirmed by restriction analysis and gel electrophoresis.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:230236
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2022-10-05 11:43:57