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undergraduate thesis
Transformacija bakterije Escherichia coli plazmidima pUC-GW-RadH i pUC-GW-NoxV
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:383323
Habek, Erik
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Habek, E. (2022). Transformacija bakterije Escherichia coli plazmidima pUC-GW-RadH i pUC-GW-NoxV (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:383323

Habek, Erik. "Transformacija bakterije Escherichia coli plazmidima pUC-GW-RadH i pUC-GW-NoxV." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:383323

Habek, Erik. "Transformacija bakterije Escherichia coli plazmidima pUC-GW-RadH i pUC-GW-NoxV." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:383323

Habek, E. (2022). 'Transformacija bakterije Escherichia coli plazmidima pUC-GW-RadH i pUC-GW-NoxV', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:383323

Habek E. Transformacija bakterije Escherichia coli plazmidima pUC-GW-RadH i pUC-GW-NoxV [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 December 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:383323

E. Habek, "Transformacija bakterije Escherichia coli plazmidima pUC-GW-RadH i pUC-GW-NoxV", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:383323

Metadata
TitleTransformacija bakterije Escherichia coli plazmidima pUC-GW-RadH i pUC-GW-NoxV
Title (english)Transformation of bacteria Escherichia coli with pUC-GW-RadH AND pUC-GW-NoxV plasmids
AuthorErik Habek
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2022-07-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovog rada bio je transformirati bakteriju Escherichia coli plazmidima pUC-GW-RadH i pUC-GW-NoxV, te potvrditi uspješnost transformacije restrikcijskom analizom plazmidne DNA izolirane iz odabranih transformanata. Navedeni plazmidi nose gene koji kodiraju za R-specifičnu alkohol-dehidrogenazu i NADH-oksidazu. Bakterija E. coli je transformirana i pla-zmidom pLS poznate koncentracije kako bi se odredila uspješnost transformacije. Iz nasumi-čno odabranih transformanata dobivenih transformacijom plazmidima pUC-GW-RadH i pUC-GW-NoxV izolirana je plazmidna DNA. Uspješnost izolacije ovih plazmida provjerena je a-garoznom gel elektroforezom, a izolirana DNA podvrgnuta je restrikcijskoj analizi kojom je potvrđeno da je bakterija E. coli uspješno transformirana plazmidima pUC-GW-RadH i pUC-GW-NoxV.
Abstract (english)
The aim of this study was to transform bacteria Escherichia coli with plasmids pUC-GW-RadH and pUC-GW-NoxV, and to confirm the transformation success by restriction analysis of plas-mid DNA isolated from selected transformants. The plasmids carry genes encoding for R-spe-cific alcohol-dehydrogenase and NADH-oxidase. Bacteria E. coli was additionally transformed with the plasmid pLS of known concentration to determine the transformation efficiency. Plas-mid DNA was isolated from randomly selected transformants obtained by transformation using plasmids pUC-GW-RadH and pUC-GW-NoxV. The success of the isolation was confirmed by agarose gel electrophoresis. The isolated plasmid DNA was subjected to restriction analysis, which confirmed that bacteria E. coli was successfully transformed with plasmids pUC-GW-RadH and pUC-GW-NoxV.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:383323
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2022-10-21 12:43:54