prikaz prve stranice dokumenta Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka
  Download
PDF 5.97 MB
doctoral thesis
Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:409424
Božić Luburić, Đurđica
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Božić Luburić, Đ. (2022). Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:409424

Božić Luburić, Đurđica. "Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:409424

Božić Luburić, Đurđica. "Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:409424

Božić Luburić, Đ. (2022). 'Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka', Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:409424

Božić Luburić Đ. Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:409424

Đ. Božić Luburić, "Određivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka", Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:409424

Metadata
TitleOdređivanje ostataka nesteroidnih protuupalnih lijekova i in vitro procjena njihova ekotoksikološkoga učinka
Title (english)Determination of nonsteroidal anti-inflammatory drug residues and in vitro assessment of their ecotoxicological impact
AuthorĐurđica Božić Luburić
MentorIvana Radojčić Redovniković (mentor)
MentorNina Bilandžić (komentor)
Committee memberKristina Radošević (predsjednik povjerenstva)
Committee memberVišnja Gaurina-Srček (član povjerenstva)
Committee memberIvana Varenina (član povjerenstva)
Committee memberIvana Kmetič (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2022-03-25, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Universal decimal classification
(UDC)		54 - Chemistry. Crystallography. Mineralogy
Abstract
U okviru doktorske disertacije razvijena je metoda za kvantitativno određivanje ostataka 27 nesteroidnih protuupalnih lijekova, kemijski različitih spojeva, tekućinskom kromatografijom ultra visoke djelotvornosti povezane s masenom spektrometrijom u uzorcima hrane životinjskog podrijetla (mlijeku, mišićnom tkivu) i uzorcima vode iz okoliša (otpadna voda). Optimiziran postupak ekstrakcije iz složenih matrica temelji se na enzimskoj hidrolizi koju slijedi ekstrakcija acetonitrilom, a dobiveni ekstrakt je pročišćen, uz istovremeno koncentriranje, ekstrakcijom na čvrstoj fazi. Validacijom metode ispitane su zadane značajke učinkovitosti koje su zadovoljile zakonski postavljene kriterije prihvatljivosti. Istraživanje je obuhvatilo i analizu realnih uzoraka s područja Republike Hrvatske, 449 uzoraka hrane životinjskog podrijetla i 96 uzoraka otpadnih voda. Kako bi se dobile informacije o toksičnosti tih spojeva koji dospijevaju u okoliš, in vitro testom na staničnim linijama ribe i ljudi ispitan je njihov citotoksični učinak.
Abstract (english)
In this dissertation, a method for the quantitative determination of 27 nonsteroidal anti-inflammatory drug residues by ultra high performance liquid chromatography – mass spectrometry in food of animal origin (milk, muscle) and environmental water (wastewater) was developed. Optimised sample processing consisted of enzymatic hydrolysis and acetonitrile extraction was followed by a clean-up of the extract by solid phase extraction. By validation of the method, verified performance characteristics complied with the legally set acceptability criteria. The study also included the analysis of the real samples from regions of the Republic of Croatia, 449 samples of food of animal origin and 96 samples of wastewater. In order to obtain information on the toxicity of these compounds that are released into the environment, their cytotoxic effect was examined using in vitro test on fish and human cell lines.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:409424
Promotion2022
Study programmeTitle: Postgraduate PhD Study in Nutrition
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam (doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam)
Type of resourceText
Extent133 str. ; 30 cm
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2022-11-09 12:46:17