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undergraduate thesis
Lipazom katalizirana kinetička (dinamička) rezolucija (R,S)-1-feniletanola u hidrofobnim niskotemperaturnim eutektičkim otapalima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:221427
Gregur, Paola
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Gregur, P. (2022). Lipazom katalizirana kinetička (dinamička) rezolucija (R,S)-1-feniletanola u hidrofobnim niskotemperaturnim eutektičkim otapalima (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:221427

Gregur, Paola. "Lipazom katalizirana kinetička (dinamička) rezolucija (R,S)-1-feniletanola u hidrofobnim niskotemperaturnim eutektičkim otapalima." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:221427

Gregur, Paola. "Lipazom katalizirana kinetička (dinamička) rezolucija (R,S)-1-feniletanola u hidrofobnim niskotemperaturnim eutektičkim otapalima." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:221427

Gregur, P. (2022). 'Lipazom katalizirana kinetička (dinamička) rezolucija (R,S)-1-feniletanola u hidrofobnim niskotemperaturnim eutektičkim otapalima', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:221427

Gregur P. Lipazom katalizirana kinetička (dinamička) rezolucija (R,S)-1-feniletanola u hidrofobnim niskotemperaturnim eutektičkim otapalima [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2025 February 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:221427

P. Gregur, "Lipazom katalizirana kinetička (dinamička) rezolucija (R,S)-1-feniletanola u hidrofobnim niskotemperaturnim eutektičkim otapalima", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:221427

Metadata
TitleLipazom katalizirana kinetička (dinamička) rezolucija (R,S)-1-feniletanola u hidrofobnim niskotemperaturnim eutektičkim otapalima
Title (english)Lipase-catalyzed kinetic (dynamic) resolution (R,S)-1-phenylethanol in hydrophobic deep eutectic solvents
AuthorPaola Gregur
MentorManuela Panić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2022-07-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Razvojem programa zelene kemije, tendencija znanstvenika i akademske zajednice je da se načela zelene kemije primjenjuju u kemijskim i biološkim procesima. Također, jedan od ciljeva je zamijeniti organska otapala alternativnim zelenim otapalima. Niskotemperaturna eutektička otapala dosad su pokazala velik potencijal za njihovu primjenu u biotransformacijama. Jedna od primjena je reakcijski medij za proizvodnju kiralnih lijekova. Prema tome, u ovom radu ispitana je aktivnost lipaza u niskotemperaturnim eutektičkim otapalima (hDES) te mogućnost korištenja hDES u enantioselektivnoj redukciji (R,S)-1-feniletiletanola uz primjenu lipaza kao biokatalizatora. Praćenjem parametara (X, VE, Vp), zaključeno je da je najaktivnija lipaza CALB u hDES-u Ty:C8. U tom sustavu je provedena kinetička (dinamička) rezolucija (R,S)-1-feniletanola te je na temelju dobivenih rezultata zaključeno da Ty:C8 moguća zamjena za organska otapala u ispitanim reakcijskim uvjetima.
Abstract (english)
With the development of the green chemistry program, the tendency of scientists and the academic community is to apply the principles of green chemistry in chemical and biological processes. Also, one of the goals is to replace organic solvents with alternative green solvents. Deep eutectic solvents have so far shown great potential for their application in biotransformations. They can be used as a reaction medium for the production of chiral drugs. Therefore, in this work, the activity of lipases in deep eutectic solvents (hDES) and the possibility of using hDES in the enantioselective reduction of (R,S)-1-phenylethylethanol with the use of lipase as a biocatalyst were tested. By monitoring the parameters (X, VE, Vp), it was concluded that the most active lipase is CALB in hDES Ty:C8. In this system, the kinetic (dynamic) resolution of (R,S)-1-phenylethanol was performed, and based on the obtained results, it was concluded that Ty:C8 is a possible substitute for organic solvents in the tested reaction conditions.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:221427
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2022-11-15 11:27:37