prikaz prve stranice dokumenta Proizvodnja lipida pomoću plijesni Mortierella isabellina
Access restricted to students and staff of home institution
master's thesis
Proizvodnja lipida pomoću plijesni Mortierella isabellina
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:087832
Ambruš, Nenad
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Ambruš, N. (2016). Proizvodnja lipida pomoću plijesni Mortierella isabellina (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:087832

Ambruš, Nenad. "Proizvodnja lipida pomoću plijesni Mortierella isabellina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:087832

Ambruš, Nenad. "Proizvodnja lipida pomoću plijesni Mortierella isabellina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:087832

Ambruš, N. (2016). 'Proizvodnja lipida pomoću plijesni Mortierella isabellina', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:087832

Ambruš N. Proizvodnja lipida pomoću plijesni Mortierella isabellina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:087832

N. Ambruš, "Proizvodnja lipida pomoću plijesni Mortierella isabellina", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:087832

Metadata
TitleProizvodnja lipida pomoću plijesni Mortierella isabellina
Title (english)Lipid production by fungus Mortierella isabellina
AuthorNenad Ambruš
MentorMirela Ivančić - Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2016-07-21, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Mikrobni lipidi su alternativna sirovina za proizvodnju biodizela druge generacije. Mortierella isabellina uzgojena je u hranjivoj podlozi uz različite molarne omjere izvora ugljika i dušika (C:N). Najveća produktivnost sinteze biomase bez lipida od 0,0285 g L-1 h-1 postignuta je pri omjeru C:N 50-150 mol mol-1. Maksimalni udjel lipida (50 %, g g-1) izmjeren je pri omjerima 150-200 mol mol-1. Kako bi se povećao prinos lipida kultura plijesni uzgojena je šaržnim postupkom uz pritok supstrata u tikvicama i bioreaktoru s miješalom (STR) primjenom dva načina prihrane izvorom ugljika i dušika. U tikvicama kultura je prihranjivana s podlogom u kojoj je omjer C:N bio 10-50 mol mol-1. U STR, kultura je prihranjivana s glukozom tako da je koncentracija dodane glukoze iznosila 40 g L-1. pH kulture održavan je dodatkom amonijaka koji je služio i kao izvor dušika za rast stanica. Primjenom ove strategije prihrane postignuta je velika produktivnost sinteze biomase bez lipida od 0,304 g L h-1 i vrlo mala produktivnost biosinteze lipida.
Abstract (english)
Microbial lipids are an alternative feedstock for production of second generation biodiesel. Mortierella isabellina was cultivated in a medium at different carbon to nitrogen molar ratios (C:N). The highest productivity of lipid-free biomass of 0,0285 g L-1 h-1 was occurred at an initial C:N ratio of 50-150 mol mol-1. Maximal lipid content of 50 % (wt/wt) was obtained at C:N ratio of 150-200 mol mol-1. In order to improve lipid yield, the fungus was grown in a fed-batch cultures in shake flasks and stirred tank reactor (STR) applying two feeding strategies with carbon and nitrogen source. In shake-flasks experiment the culture was fed with media at 10-50 mol mol-1 C:N ratio. In STR, the culture was fed by glucose to give 40 g L-1. pH was maintained using ammonia, which was also used as a nitrogen source for cell growth. Using fed batch strategy, high productivity of lipid free-biomass of 0,304 g/L/h and low lipid productivity was obtained.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:087832
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-27 09:58:57