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master's thesis
Optimizacija sustava za izlaganje heterolognih proteina na površini stanica kvasca putem proteina Pir2
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:551068
Matičević, Ana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Matičević, A. (2022). Optimizacija sustava za izlaganje heterolognih proteina na površini stanica kvasca putem proteina Pir2 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:551068

Matičević, Ana. "Optimizacija sustava za izlaganje heterolognih proteina na površini stanica kvasca putem proteina Pir2." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:551068

Matičević, Ana. "Optimizacija sustava za izlaganje heterolognih proteina na površini stanica kvasca putem proteina Pir2." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:551068

Matičević, A. (2022). 'Optimizacija sustava za izlaganje heterolognih proteina na površini stanica kvasca putem proteina Pir2', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:551068

Matičević A. Optimizacija sustava za izlaganje heterolognih proteina na površini stanica kvasca putem proteina Pir2 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:159:551068

A. Matičević, "Optimizacija sustava za izlaganje heterolognih proteina na površini stanica kvasca putem proteina Pir2", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:551068

Metadata
TitleOptimizacija sustava za izlaganje heterolognih proteina na površini stanica kvasca putem proteina Pir2
Title (english)Optimisation of yeast surface display using protein Pir2
AuthorAna Matičević
MentorRenata Teparić (mentor)
Committee memberVladimir Mrša (predsjednik povjerenstva)
Committee memberIrena Landeka Jurčević (član povjerenstva)
Committee memberBojan Žunar (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2022-07-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Metoda izlaganja heterolognih proteina na površini stanice kvasca Saccharomyces cerevisiae alternativa je klasičnim metodama imobilizacije proteina, a temelji se na fuziji gena koji kodira za protein od interesa sa genom koji kodira za neki od proteina stanične stijenke. Optimizacija sustava za izlaganje proteina na površini stanice kvasca u ovome je radu provedena uvođenjem promjena u sekvencu proteina Pir2 te promjenom pozicije proteina od interesa unutar sekvence proteina Pir2. U promatranom sustavu mjerenjem aktivnosti ˝reporter˝ enzima β-laktamaze te metodom imunoblota proteina stanične stijenke procijenjena je količina rekombinantnog proteina Pir2bla u staničnoj stijenki. Navedenim metodama promatran je i utjecaj mutacija u endocitozi, korištenjem mutanata end3 i end6, na efikasnost izlaganja rekombinantnog proteina na površini stanice u svrhu daljnje optimizacije sustava za imobilizaciju.
Abstract (english)
Surface display of enzymes at the cell wall of yeast Saccharomyces cerevisiae presents an alternative to chemical immobilization methods. Methods for yeast surface display are based on the fusion of a gene encoding a protein of interest with a gene encoding one of the cell wall proteins. Optimization of the protein surface display system in this paper was performed by introducing changes in the Pir2 protein sequence and changing the position of the reporter protein within the Pir2 protein sequence. In the observed system, the amount of recombinant Pir2bla protein in the cell wall was estimated by measuring the activity of the reporter enzyme β-lactamase and the immunoblot of yeast cell wall proteins. Before mentioned methods were also used to investigate the impact of mutations in endocytosis, by using mutants end3 and end6, on efficiency of surface display system in order to optimize immobilization method.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:551068
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2023-01-19 12:23:05