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master's thesis
Istraživanje učinkovitosti transfekcije CHO DP-12 stanica u mediju obogaćenom proteinskim hidrolizatima lana
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:003455
Kozarić, Vlatka
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Kozarić, V. (2022). Istraživanje učinkovitosti transfekcije CHO DP-12 stanica u mediju obogaćenom proteinskim hidrolizatima lana (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:003455

Kozarić, Vlatka. "Istraživanje učinkovitosti transfekcije CHO DP-12 stanica u mediju obogaćenom proteinskim hidrolizatima lana." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:003455

Kozarić, Vlatka. "Istraživanje učinkovitosti transfekcije CHO DP-12 stanica u mediju obogaćenom proteinskim hidrolizatima lana." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:003455

Kozarić, V. (2022). 'Istraživanje učinkovitosti transfekcije CHO DP-12 stanica u mediju obogaćenom proteinskim hidrolizatima lana', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 17 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:003455

Kozarić V. Istraživanje učinkovitosti transfekcije CHO DP-12 stanica u mediju obogaćenom proteinskim hidrolizatima lana [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 November 17] Available at: https://urn.nsk.hr/urn:nbn:hr:159:003455

V. Kozarić, "Istraživanje učinkovitosti transfekcije CHO DP-12 stanica u mediju obogaćenom proteinskim hidrolizatima lana", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:003455

Metadata
TitleIstraživanje učinkovitosti transfekcije CHO DP-12 stanica u mediju obogaćenom proteinskim hidrolizatima lana
Title (english)Investigation of the efficiency of CHO DP-12 cell transfection in media enriched with flaxseed protein hydrolysates
AuthorVlatka Kozarić
MentorIgor Slivac (mentor)
Committee memberVišnja Gaurina-Srček (predsjednik povjerenstva)
Committee memberAndreja Leboš-Pavunc (član povjerenstva)
Committee memberTeuta Murati (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
Zagreb
Defense date and country2022-07-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Interdisciplinary Technical Sciences
Abstract
U posljednje vrijeme sve je veći interes za primjenom uljnih pogača sjemenki lana zbog njihovog visoko vrijednog sastava u kojem oko 20 % čine proteini. Kako je već ranije utvrđeno da proteinski hidrolizati lana potiču rast CHO DP-12 stanica, u ovom radu se otišlo korak dalje te je ispitan njihov utjecaj na uspješnost transfekcije CHO DP-12 stanica. Transfekcija se provodila i na adherentnim i na suspenzijskim stanicama. Korištena su dva transfekcijska sredstva, FuGene 6 i Turbofect te nekoliko različitih hranjivih medija (PowerCHO, DMEM, ExCell, CD CHO, FidCHO). Transfekcija je zabilježena samo u DMEM mediju uz FuGene 6 transfekcijsko sredstvo, bez dodatka hidrolizata lana. Ispitivanjem različitih omjera mase plazmida (μg) i transfekcijskog sredstva (mL), ustanovljeno je da najveću učinkovitost transfekcije daje omjer 1:6. Iako je utvrđeno da proteinski hidrolizati lana imaju negativan utjecaj na transfekciju CHO DP-12 stanica, dobiveni rezultati su dobra podloga za daljnja istraživanja u cilju pronalaska optimalnih uvjeta transfekcije.
Abstract (english)
The application of flaxseed oil cake attracts great interest due to its highly valuable composition and protein content of about 20 %. As it has been already known that flax protein hydrolysates stimulate the growth of CHO DP-12 cells, this work went a step further and their impact on the efficiency of CHO DP-12 cells transfection has been examined. Transfection was performed on both adherent and suspension cells. Two transfection reagents were used, FuGene 6 and Turbofect, and several different nutrient media (PowerCHO, DMEM, ExCell, CD CHO, FidCHO). Transfection was recorded only in DMEM medium with FuGene 6 transfection reagent, without adding flax hydrolyzates. Examination of different ratios of plasmid mass (μg) and transfection reagent (mL), revealed that the ratio of 1:6 gives the highest transfection efficiency. Although it has been determined that flax protein hydrolysates have a negative effect on CHO DP-12 cell transfection, the obtained results are good foundation for further research to find optimal transfection conditions.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:003455
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2023-01-19 13:19:35