prikaz prve stranice dokumenta The role of long non-coding RNAs and the RNA exosome in regulation of gene expression
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doctoral thesis
The role of long non-coding RNAs and the RNA exosome in regulation of gene expression
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:982678
Ana, Novačić
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Ana, N. (2023). The role of long non-coding RNAs and the RNA exosome in regulation of gene expression (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:982678

Ana, Novačić. "The role of long non-coding RNAs and the RNA exosome in regulation of gene expression." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:982678

Ana, Novačić. "The role of long non-coding RNAs and the RNA exosome in regulation of gene expression." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:982678

Ana, N. (2023). 'The role of long non-coding RNAs and the RNA exosome in regulation of gene expression', Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:982678

Ana N. The role of long non-coding RNAs and the RNA exosome in regulation of gene expression [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 July 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:982678

N. Ana, "The role of long non-coding RNAs and the RNA exosome in regulation of gene expression", Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:982678

Metadata
TitleThe role of long non-coding RNAs and the RNA exosome in regulation of gene expression
Title (croatian)Uloga dugih nekodirajućih molekula RNA i RNA egzosoma u regulaciji ekspresije gena
AuthorNovačić Ana
MentorIgor Stuparević (mentor)
Committee memberIvan Krešimir Svetec (predsjednik povjerenstva)
Committee memberRenata Teparić (član povjerenstva)
Committee memberMarta Popović (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2023-12-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Universal decimal classification
(UDC)		578/579 - Virology. Microbiology
Abstract
The RNA exosome is a conserved protein complex involved in 3'-to-5' degradation and processing of all types of RNA classes in eukaryotic cells. The recently characterized substrates of the RNA exosome complex are long non-coding RNAs (lncRNAs), transcripts that do not code for proteins, but have the potential to regulate gene expression. The main objective of this work was to determine the role of lncRNAs and the RNA exosome complex in the regulation of stress-related genes in yeast. This work demonstrates that the RNA exosome is critical for maintaining yeast cell wall stability by regulating the expression of genes encoding enzymes involved in protein glycosylation, which is essential for supporting the cell wall structure. In maintaining cell wall stability, the RNA exosome functions in parallel with Ssd1, an RNA-binding protein that regulates translation of cell wall-related mRNAs. Furthermore, this work elucidates the molecular mechanisms through which lncRNAs and the RNA exosome regulate the expression of stress-related genes. A regulatory role has been demonstrated for the lncRNA transcribed at the gene locus encoding the protein Psa1, which is involved in the synthesis of the mannosylation precursor. Furthermore, this work demonstrates a negative regulatory role of antisense transcription at the PHO5 model gene locus at the level of promoter chromatin structure.
Abstract (croatian)
RNA egzosom je evolucijski očuvani proteinski kompleks uključen u razgradnju i procesiranje svih klasa RNA u smjeru 3'-5' u eukariotskim stanicama. Nedavno karakterizirani supstrati RNA egzosoma su duge nekodirajuće molekule RNA (lncRNA), transkripti koji ne nose uputu za sintezu proteina, ali imaju potencijalne uloge u regulaciji ekspresije gena. Glavni cilj ovog rada bio je odrediti ulogu lncRNA i kompleksa RNA egzosoma u regulaciji ekspresije gena kvasca čija je funkcija povezana sa stresom. Ovaj rad pokazuje da je RNA egzosom ključan za održavanje stabilnosti stanične stijenke kvasca, budući da regulira ekspresiju gena koji kodiraju za enzime uključene u glikozilaciju proteina, koja je bitna za održavanje strukture stanične stijenke. U održavanju stabilnosti stanične stijenke, RNA egzosom funkcionira paralelno sa Ssd1, RNA-vezujućim proteinom koji regulira translaciju mRNA koje nose upute za proteine uključene u staničnu morfogenezu. Nadalje, ovaj rad razjašnjava molekularne mehanizme putem kojih lncRNA i RNA egzosom reguliraju ekspresiju gena povezanih sa stresom. Pokazana je regulatorna uloga lncRNA transkribirane na lokusu gena koji kodira za protein Psa1, koji je uključen u sintezu manozilacijskog prekursora. Dodatno, ovim je radom pokazana negativna regulatorna ulogu antisense transkripcije na lokusu modelnog gena PHO5 na razini strukture kromatina promotorske regije.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:982678
Promotion2023
Study programmeTitle: Postgraduate PhD Study in Nutrition
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam (doktor/doktorica znanosti, područje biotehničkih znanosti, polje nutricionizam)
Type of resourceText
Extent106 str. : 30 cm
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2023-01-23 11:11:24