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master's thesis
Uloga Rab proteina u uspješnosti transdukcije adenovirusom tipa 26
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:754444
Kašnar, Klara
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Kašnar, K. (2022). Uloga Rab proteina u uspješnosti transdukcije adenovirusom tipa 26 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:754444

Kašnar, Klara. "Uloga Rab proteina u uspješnosti transdukcije adenovirusom tipa 26." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:754444

Kašnar, Klara. "Uloga Rab proteina u uspješnosti transdukcije adenovirusom tipa 26." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:754444

Kašnar, K. (2022). 'Uloga Rab proteina u uspješnosti transdukcije adenovirusom tipa 26', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:754444

Kašnar K. Uloga Rab proteina u uspješnosti transdukcije adenovirusom tipa 26 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 December 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:754444

K. Kašnar, "Uloga Rab proteina u uspješnosti transdukcije adenovirusom tipa 26", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:754444

Metadata
TitleUloga Rab proteina u uspješnosti transdukcije adenovirusom tipa 26
Title (english)The role of Rab protiens in the adenovitus type 26 transduction
AuthorKlara Kašnar
MentorKsenija Durgo (mentor)
Committee memberMarina Cvjetko Bubalo (predsjednik povjerenstva)
Committee memberDragomira Majhen (član povjerenstva)
Committee memberIgor Stuparević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2022-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Rekombinantni adenovirusni vektori koriste se kao viralni vektori u genskoj terapiji i vakcinaciji. Najčešće korišten adenovirusni vektor temeljen je na adenovirusu tipa 5 (HAdV-C5) čija je uporaba ograničena zbog visoke seroprevalencije pa se sve više istražuju manje seroprevalentni adenovirusi kao što je adenovirus tipa 26 (HAdV-D26). Rab proteini imaju brojne uloge u stanici, a smatraju se ključnim regulatorima staničnog transporta. U ovom radu ispitana je uloga Rab5, Rab7, Rab9 i Rab11 proteina u endocitozi i unutarstaničnom putovanju HAdV-D26. Stanice su transficirane plazmidima koji sadrže gene za Rab proteine divljeg tipa (WT, engl. wild type) te je uspješnost transdukcije tih stanica uspoređena s uspješnošću transdukcije stanica transficiranih plazmidima koji sadrže gene za dominantno negativne (DN, engl. dominant negative) mutante Rab proteina. Zabilježena je manja uspješnost transdukcije u stanicama transficiranima s plazmidima koji kodiraju za DN mutante Rab proteina što ukazuje na ulogu Rab proteina u endocitozi i unutarstaničnom putovanju HAdV-D26.
Abstract (english)
Recombinant adenoviral vectors are used as viral vectors in gene therapy and vaccination. The most used adenovirus vector is based on adenovirus type 5 (HAdV-C5), but its use is limited due to the high seroprevalence thus, less seroprevalent adenoviruses such as adenovirus type 26 (HAdV-D26) are investigated as alternative. Rab proteins have numerous roles in the cell and are considered key regulators of cellular transport. In this study we investigatedthe role of Rab5, Rab7, Rab9 and Rab11 proteins in endocytosis and intracellular trafficking of HAdV-D26. HAdV-D26 transduction was compared in cells transfected with plasmids containing genes for wild-type (WT) Rab proteins and cells transfected with plasmids containing genes for dominant negative (DN) mutants of Rab proteins. A lower transduction efficiency was observed in cells transfected with plasmids encoding dominant negative mutants indicating the role of Rab proteins in endocytosis and intracellular trafficking of HAdV-D26.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:754444
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2023-02-13 14:44:15