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master's thesis
Ekstrakcija kanabidiola s hidrofobnim niskotemperaturnim eutektičkim otapalima i učinci u uvjetima in vitro na stanične linije
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:341843
Češi, Martin
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Engineering
Laboratory for Oil and Fat Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Češi, M. (2022). Ekstrakcija kanabidiola s hidrofobnim niskotemperaturnim eutektičkim otapalima i učinci u uvjetima in vitro na stanične linije (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:341843

Češi, Martin. "Ekstrakcija kanabidiola s hidrofobnim niskotemperaturnim eutektičkim otapalima i učinci u uvjetima in vitro na stanične linije." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:341843

Češi, Martin. "Ekstrakcija kanabidiola s hidrofobnim niskotemperaturnim eutektičkim otapalima i učinci u uvjetima in vitro na stanične linije." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:341843

Češi, M. (2022). 'Ekstrakcija kanabidiola s hidrofobnim niskotemperaturnim eutektičkim otapalima i učinci u uvjetima in vitro na stanične linije', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 13 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:341843

Češi M. Ekstrakcija kanabidiola s hidrofobnim niskotemperaturnim eutektičkim otapalima i učinci u uvjetima in vitro na stanične linije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 July 13] Available at: https://urn.nsk.hr/urn:nbn:hr:159:341843

M. Češi, "Ekstrakcija kanabidiola s hidrofobnim niskotemperaturnim eutektičkim otapalima i učinci u uvjetima in vitro na stanične linije", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:341843

Metadata
TitleEkstrakcija kanabidiola s hidrofobnim niskotemperaturnim eutektičkim otapalima i učinci u uvjetima in vitro na stanične linije
Title (english)Extraction of cannabidiol with hydrophobic low-temperature eutectic solvents and effects in vitro on cell lines
AuthorMartin Češi
MentorMarko Obranović (mentor)
MentorAndreja Jurič (komentor)
Committee memberIvana Radojčić Redovniković (predsjednik povjerenstva)
Committee memberNevenka Kopjar (član povjerenstva)
Committee memberSandra Balbino (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Food Engineering)
(Laboratory for Oil and Fat Technology)
Zagreb
Defense date and country2022-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Ekstrakcija kanabinoida, primarno kanabidiola (CBD), iz industrijske konoplje je relativno nova grana industrije i ostavlja dovoljno prostora za inovacije i optimiranje procesa. Standardni procesi uključuju primjenu etanola i drugih organskih otapala te superkritičnog CO2. Kao alternativa ovim postupcima ispitana je mogućnost primjene lipofilnih eutektičkih otapala (DES). U radu su napravljena preliminarna laboratorijska istraživanja i optimiranje procesa na odabranom DES-u uz varijacije omjera suhe tvari i otapala, vremena ekstrakcije te temperature. Udio ekstrahiranih ukupnih kanabinoida, CBD i kanabinola (CBN) određen je putem tekućinske kromatografije dok je udio nepoželjnih klorofila u ekstraktu određen spektrofotometrijski. Provedeni su toksikološki testovi in vitro na staničnim linijama, koji pokazuju povoljne učinke na stanično preživljenje. Time je dokazano da ekstrakt CBD-a u hidrofobnom DES-u ima značajan potencijal za daljna istraživanja i moguću primjenu u prehrambenoj industriji, farmciji i kozmetici
Abstract (english)
The extraction of cannabinoids, primarily cannabidiol (CBD), from industrial hemp is a relatively new branch of the industry and leaves plenty of room for innovation and optimization of the process. Standard processes include the use of ethanol and other organic solvents and supercritical CO2. As an alternative to these procedures, the possibility of using lipophilic eutectic solvents (DES) was examined. In the paper, preliminary laboratory research and optimization of the process on the selected DES were carried out with variations in the ratio of dry matter and solvent, extraction time and temperature. The proportion of extracted total cannabinoids, CBD and cannabinol (CBN) was determined by liquid chromatography, while the proportion of undesirable chlorophylls in the extract was determined spectrophotometrically. In vitro toxicological tests were performed on cell lines, which show favorable effects on cell survival and genome stability. This proved that CBD extract in hydrophobic DES has signifivant potential for further research and possible application in the food industry, pharmacy and cosmetics.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:341843
Study programmeTitle: Food Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2023-02-14 14:18:39