prikaz prve stranice dokumenta Mejotička regulacija razine proteina Rrp6 proteolitičkim procesiranjem i nekodirajućom rna u stanicama kvasca Saccharomyces cerevisiae
  Download
PDF 2.61 MB
master's thesis
Mejotička regulacija razine proteina Rrp6 proteolitičkim procesiranjem i nekodirajućom rna u stanicama kvasca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:141394
Štrbac, Lucija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Štrbac, L. (2022). Mejotička regulacija razine proteina Rrp6 proteolitičkim procesiranjem i nekodirajućom rna u stanicama kvasca Saccharomyces cerevisiae (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:141394

Štrbac, Lucija. "Mejotička regulacija razine proteina Rrp6 proteolitičkim procesiranjem i nekodirajućom rna u stanicama kvasca Saccharomyces cerevisiae." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:141394

Štrbac, Lucija. "Mejotička regulacija razine proteina Rrp6 proteolitičkim procesiranjem i nekodirajućom rna u stanicama kvasca Saccharomyces cerevisiae." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:141394

Štrbac, L. (2022). 'Mejotička regulacija razine proteina Rrp6 proteolitičkim procesiranjem i nekodirajućom rna u stanicama kvasca Saccharomyces cerevisiae', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 04 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:141394

Štrbac L. Mejotička regulacija razine proteina Rrp6 proteolitičkim procesiranjem i nekodirajućom rna u stanicama kvasca Saccharomyces cerevisiae [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 July 04] Available at: https://urn.nsk.hr/urn:nbn:hr:159:141394

L. Štrbac, "Mejotička regulacija razine proteina Rrp6 proteolitičkim procesiranjem i nekodirajućom rna u stanicama kvasca Saccharomyces cerevisiae", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:141394

Metadata
TitleMejotička regulacija razine proteina Rrp6 proteolitičkim procesiranjem i nekodirajućom rna u stanicama kvasca Saccharomyces cerevisiae
Title (english)Meiotic regulation of Rrp6 protein level by proteolytic processing and non-coding RNA in yeast Saccharomyces cerevisiae
AuthorLucija Štrbac
MentorIgor Stuparević (mentor)
Committee memberRenata Teparić (predsjednik povjerenstva)
Committee memberMarina Svetec Miklenić (član povjerenstva)
Committee memberIgor Slivac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2022-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
RNA egzosom je multiproteinski kompleks ključan za kontrolu kvalitete RNA u svim eukariotskim stanicama. Egzoribonukleazna podjedinica RNA egzosoma Rrp6 esencijalna je za mejozu i sporulaciju kvasca S. cerevisiae, međutim razina proteina Rrp6 smanjuje se tijekom mejoze. U ovom je radu pokazan proteolitički mehanizam regulacije razine proteina Rrp6 kompleksom koji promovira anafazu/ciklosom (APC/C) i proteasomom. Rezultati su pokazali kako se u uvjetima mejoze razina Rrp6 stabilizira u mutantu kvasca u kojem je onemogućeno vezanje APC/C na Rrp6 te u uvjetima rasta u kojima je proteasom inhibiran. Također, degradacija Rrp6 tijekom mejoze dovodi do stabilizacije nekodirajućih RNA transkripata (MUT) kojima su uloge slabo istražene, zbog čega je razmotrena i potencijalna regulatorna uloga antisense transkripta s lokusa RRP6, MUT1312, u regulaciji ekspresije Rrp6. Ovim radom istražen je i fiziološki značaj degradacije Rrp6 tijekom mejoze, pri čemu je pokazano da zadržavanje visoke razine proteina Rrp6 tijekom mejoze ima negativan učinak na tijek sporulacije.
Abstract (english)
The RNA exosome is a multiprotein complex crucial for RNA quality control in all eukaryotic cells. The exoribonuclease subunit of RNA exosome Rrp6 is essential for meiosis and sporulation in the yeast S. cerevisiae, however Rrp6 protein level decreases during meiosis. This work demonstrates that Rrp6 level is regulated through targeted proteolysis by the anaphase promoting complex/cyclosome (APC/C) and the proteasome. The results show that Rrp6 is stabilized in meiotic conditions when the binding site of APC/C is mutated or when the proteasome is inhibited. As degradation of Rrp6 during meiosis results in stabilization of non-coding RNA transcripts (MUTs) with poorly characterized roles, this work explored the potential role of the antisense RRP6 transcript, MUT1312, in regulation of Rrp6 expression. The physiological significance of Rrp6 degradation was also examined, showing that maintaining high Rrp6 protein levels throughout meiosis had a negative effect on sporulation kinetics.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:141394
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2023-02-24 13:25:22