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master's thesis
In vitro adhezija bakterija mliječne kiseline iz majčinog mlijeka
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:327279
Ribić, Valentina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Ribić, V. (2022). In vitro adhezija bakterija mliječne kiseline iz majčinog mlijeka (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:327279

Ribić, Valentina. "In vitro adhezija bakterija mliječne kiseline iz majčinog mlijeka." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:327279

Ribić, Valentina. "In vitro adhezija bakterija mliječne kiseline iz majčinog mlijeka." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:327279

Ribić, V. (2022). 'In vitro adhezija bakterija mliječne kiseline iz majčinog mlijeka', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:327279

Ribić V. In vitro adhezija bakterija mliječne kiseline iz majčinog mlijeka [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:159:327279

V. Ribić, "In vitro adhezija bakterija mliječne kiseline iz majčinog mlijeka", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:327279

Metadata
TitleIn vitro adhezija bakterija mliječne kiseline iz majčinog mlijeka
Title (english)In vitro adhesion of lactic acid bacteria from human breast milk
AuthorValentina Ribić
MentorAndreja Leboš Pavunc (mentor)
Committee memberBlaženka Kos (predsjednik povjerenstva)
Committee memberJasna Mrvčić (član povjerenstva)
Committee memberKsenija Durgo (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2022-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovog rada bio je in vitro ispitati adhezijska i probiotička svojstva sojeva bakterija mliječne kiseline izoliranih iz majčinog mlijeka. Ispitana su svojstva sojeva Levilactobacillus brevis MB13 i MB20 kroz eksperimente adhezije na Caco-2 stanice i na proteine ekstracelularnog matriksa te ispitivanjem preživljavanja u simuliranim uvjetima gastrointestinalnog trakta (GIT) pri čemu je istovremeno ispitana i uloga S-proteina u adheziji i zaštiti od nepovoljnih uvjeta. Također, eksperimentom kompetitivne ekskluzije ispitano je djelovanje sojeva L. brevis MB13 i MB20, Limosilactobacillus fermentum MC1 te Lactiplantibacillus plantarum MC19 i KR19 prema patogenoj vrsti Salmonella enterica serovar Typhimurium FP1. Provedenim eksperimentima dokazano je da sojevi L. brevis MB13 i MB20 posjeduju adhezijska svojstva i mogu preživjeti nepovoljne uvjete GIT-a, međutim za to je odgovoran S-sloj proteina čije uklanjanje uzrokuje značajno smanjenje adhezije te nemogućnost preživljavanja u GIT-u. Također, kompetitivnom ekskluzijom dokazano je da svi ispitani sojevi imaju antimikrobno djelovanje prema S. enterica serovar Typhimurium FP1.
Abstract (english)
The aim of this work was to test in vitro the adhesion and probiotic properties of lactic acid bacteria strains isolated from human breast milk. The properties of Levilactobacillus brevis strains MB13 and MB20 were tested through experiments of adhesion on Caco-2 cells and extracellular matrix proteins and survival tests in simulated conditions of the gastrointestinal tract (GIT), at the same time the role of S-protein in adhesion and protection against adverse conditions was tested. Also, the activity of L. brevis MB13 and MB20, Limosilactobacillus fermentum MC1, Lactiplantibacillus plantarum MC19 and KR19 strains against the pathogenic species Salmonella enterica serovar Typhimurium FP1 was tested using a competitive exclusion experiment. The conducted experiments proved that L. brevis strains MB13 and MB20 possess adhesive properties and can survive unfavorable conditions of the GIT, however, the S-layer protein is responsible for this, the removal of which causes a significant reduction in adhesion and the inability to survive in the GIT. Also, competitive exclusion proved that all tested strains have antimicrobial activity against S. enterica serovar Typhimurium FP1.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:327279
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2023-03-15 12:13:37