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master's thesis
Utjecaj mutacija proteina Keap1 na interakciju s dipeptidil-peptidazom III
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:130918
Kolak, Martina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Kolak, M. (2022). Utjecaj mutacija proteina Keap1 na interakciju s dipeptidil-peptidazom III (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:130918

Kolak, Martina. "Utjecaj mutacija proteina Keap1 na interakciju s dipeptidil-peptidazom III." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:130918

Kolak, Martina. "Utjecaj mutacija proteina Keap1 na interakciju s dipeptidil-peptidazom III." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:130918

Kolak, M. (2022). 'Utjecaj mutacija proteina Keap1 na interakciju s dipeptidil-peptidazom III', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:130918

Kolak M. Utjecaj mutacija proteina Keap1 na interakciju s dipeptidil-peptidazom III [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:159:130918

M. Kolak, "Utjecaj mutacija proteina Keap1 na interakciju s dipeptidil-peptidazom III", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:130918

Metadata
TitleUtjecaj mutacija proteina Keap1 na interakciju s dipeptidil-peptidazom III
Title (english)Influence of Keap1 mutations on dipeptidyl peptidase III interaction
AuthorMartina Kolak
MentorKristina Radošević (mentor)
Committee memberIgor Stuparević (predsjednik povjerenstva)
Committee memberMihaela Matovina (član povjerenstva)
Committee memberIgor Slivac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2022-07-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Dipeptidil-peptidaza III osim cijepanja dipeptida s N-terminalnog kraja oligopeptida ima i ulogu u regulaciji signalnog puta Keap1-Nrf2. Nrf2 je transkripcijski faktor koji je ključan u odgovoru stanice na oksidacijska oštećenja, a vežući se na protein Keap1 usmjerava se ka ubikvitinaciji i razgradnji. DPP III, kao i Nrf2, sadrži motiv ETGE preko kojeg se ostvaruju interakcije s domenom Kelch proteina Keap1. Na internetskom portalu za genomiku raka cBioPortal nalaze se genomske sekvence mutanata Keap1 pronađene u uzorcima zloćudnih tumora. U ovom radu su proučavane dvije mutacije Keap1 proteina, G333C i G480W, te njihov utjecaj na interakciju s proteinom DPP III. Istraživanje je provedeno primjenom stanične linije HEK293T.
Abstract (english)
Dipeptidyl-peptidase III, in addition to cleaving dipeptides from the N-terminal end of oligopeptides, also plays a role in the regulation of the Keap1-Nrf2 signalling pathway. Nrf2 is a transcription facto that is crucial in the cell response to oxidative damage, and binding to the Keap1 protein directs it toward ubiquitination and degradation. Both Nrf2 and DPP III contain an ETGE motif through which the interactions with the Kelch domain of the Keap1 protein are achieved. The cancer genomics web portal cBioPortal contains genomic sequences of Keap1 mutants found in cancer samples. In this work, two mutations of the Keap1 protein, G333C and G480W, their influence on the interaction with the DPP III protein were studied. The study was performed using the HEK293T cell line.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:130918
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2023-07-21 11:44:04