The new coronavirus SARS-CoV-2 is primarily a respiratory virus, but the routes of transmission have not been fully clarified, including the one through the food chain. The ribonucleic acid (RNA) isolation technique is one of the most important elements in the detection of the SARS-CoV-2. In this paper, experimental testing and evaluation of the four commercially available extraction kits with the original protocol of the manufacturer and a modified protocol in the precipitation step in which ethanol and isopropanol were used were made. The selected kit was used to isolate RNA from swab samples of food packaging surfaces, food surfaces and ready-to-eat meals, followed by virus detection via real-time RT-PCR. The obtained results gave an insight into the actual situation of the presence of the SARS-CoV-2 virus in the food chain, and with a review of the available literature, made it possible to clarify the role and significance of food as a possible vector for the transmission of the SARS-CoV-2 virus. In addition to testing the presence of viruses, all samples were tested for bacterial flora, indicator microorganisms in swabs, and in food for microorganisms according to the criteria of the national Guide for microbiological criteria. The results of the confirmation of the applicability of the selected kit are particularly significant, indicating a significant proportion of surface samples positive for the SARS-CoV-2 virus, with the emphasis that these samples are from areas where sick persons with less pronounced symptoms or no symptoms have stayed. According to the results of the final part of the study in which the samples related to the food chain were examined, it can be concluded that the possibility of infection is trivial since the virus and virus particles were not detected in these samples.