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undergraduate thesis
Aktivnost odabranih oksidoreduktaza u niskotemperaturnim eutektičkim otapalima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:136335
Mah'D, Jasmin
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Mah'D, J. (2023). Aktivnost odabranih oksidoreduktaza u niskotemperaturnim eutektičkim otapalima (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:136335

Mah'D, Jasmin. "Aktivnost odabranih oksidoreduktaza u niskotemperaturnim eutektičkim otapalima." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:136335

Mah'D, Jasmin. "Aktivnost odabranih oksidoreduktaza u niskotemperaturnim eutektičkim otapalima." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:136335

Mah'D, J. (2023). 'Aktivnost odabranih oksidoreduktaza u niskotemperaturnim eutektičkim otapalima', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 28 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:136335

Mah'D J. Aktivnost odabranih oksidoreduktaza u niskotemperaturnim eutektičkim otapalima [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 June 28] Available at: https://urn.nsk.hr/urn:nbn:hr:159:136335

J. Mah'D, "Aktivnost odabranih oksidoreduktaza u niskotemperaturnim eutektičkim otapalima", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:136335

Metadata
TitleAktivnost odabranih oksidoreduktaza u niskotemperaturnim eutektičkim otapalima
Title (english)Activity of selected oxidoreductased in deep eutectic solvents
AuthorJasmin Mah'D
MentorMarina Cvjetko Bubalo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2023-09-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Ovim radom ispitana je aktivnost enzima alkohola dehidrogenaze (ADH) i litičke polisaharidne monooksigenaze (LPMO) u niskotemperaturnim eutektičkim otapalima (engl. Deep Eutectic Solvents, DES). Odabrane su lako dostupne, netoksične i relativno jeftine komponente za izradu niskotemperaturnih eutektičkih otapala prema načelima zelene kemije i održivog razvoja. Nakon sinteze otapala, spektrofotometrijski je ispitana aktivnost ADH u glicin pirofosfatnom puferu pH 9 (GPP) i LPMO u natrijev acetatnom puferu (NaAc) puferu pH 6 u svrhu usporedbe aktivnosti. Potom je aktivnost enzima ispitana u priređenim otapalima istom spektrofotometrijskom metodom uz pufer kao slijepu probu. Svrha rada je bila ispitati primjenu DES-ova te usporediti i eksperimentalno potkrijepiti aktivnost ADH i LPMO u pripremljenim DES-ovima
Abstract (english)
This work investigated the activity of alcohol dehydrogenase (ADH) and lytic polysaccharide monooxygenase enyzme (LPMO) in low-temperature deep eutectic solvents (DES). Easily accessible, non-toxic, and relatively inexpensive components were selected to create the deep eutectic solvents according to the principles of green chemistry and sustainable development. LPMO activity was initially spectrophotometrically tested in NaAc buffer pH 6, while ADH activity was spectrophotometrically tested in glycine pyrophosphate buffer pH 9 for comparison. Then, enzyme activity was tested in prepared solvents using the same method with buffer as a blank. Purpose of the work was to investigate the usage of DES and experimentally substantiate the activity of ADH and LPMO in prepared DES.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:136335
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-04-02 08:42:55