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master's thesis
Aktivnost litičke polisaharidne monooksigenaze u niskotemepraturnim eutektičkim otapalima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:690731
Perković, Ivan
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Perković, I. (2023). Aktivnost litičke polisaharidne monooksigenaze u niskotemepraturnim eutektičkim otapalima (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:690731

Perković, Ivan. "Aktivnost litičke polisaharidne monooksigenaze u niskotemepraturnim eutektičkim otapalima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:690731

Perković, Ivan. "Aktivnost litičke polisaharidne monooksigenaze u niskotemepraturnim eutektičkim otapalima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:690731

Perković, I. (2023). 'Aktivnost litičke polisaharidne monooksigenaze u niskotemepraturnim eutektičkim otapalima', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:690731

Perković I. Aktivnost litičke polisaharidne monooksigenaze u niskotemepraturnim eutektičkim otapalima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2025 February 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:690731

I. Perković, "Aktivnost litičke polisaharidne monooksigenaze u niskotemepraturnim eutektičkim otapalima", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:690731

Metadata
TitleAktivnost litičke polisaharidne monooksigenaze u niskotemepraturnim eutektičkim otapalima
Title (english)Activity of lytic polysaccharide monooxygenase in low-temperature deep eutectic solvents
AuthorIvan Perković
MentorTonči Rezić (mentor)
Committee memberMarina Cvjetko Bubalo (predsjednik povjerenstva)
Committee memberAna Tušek Jurinjak (član povjerenstva)
Committee memberIvana Radojčić Redovniković (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2023-05-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Ovim radom ispitana je aktivnost enzima litičke polisaharidne monooksigenaze (LPMO) u niskotemperaturnim eutektičkim otapalima (engl. Deep Eutectic Solvents, DES) te je izrađen jednostavan i robusan matematički model kojime se nastoji predviditi korištenje otapala s najprikladnijim fizikalno kemijskim svojstvima njegovih sastavnih komponenata za biokatalitičku reakciju LPMO. Odabrane su lako dostupne, netoksične i relativno jeftine komponente za izradu niskotemperaturnih eutektičkih otapala prema načelima zelene kemije i održivog razvoja. Nakon sinteze otapala, spektrofotometrijski je ispitana aktivnost LPMO prvotno u NaAc puferu pH 6,4 u svrhu usporedbe aktivnosti. Potom je aktivnost enzima ispitana u priređenim otapalima istom spektrofotometrijskom metodom uz pufer kao slijepu probu. Nadalje, DES-ovi su matematički opisani pomoću vrijednosti dobivenih COSMO-RS programskim paketom. Dobivene vrijednosti grupirane su u 10 intervala kako bi se načinio sveukupni sigma profil DES-a. Na osnovi izrađenih intervala programskim paketom Statistica 13.0 izrađeni su modeli višestruke linearne regresije te djelomične linearne regresije
Abstract (english)
This work investigated the activity of the lytic polysaccharide monooxygenase enyzme (LPMO) in low-temperature deep eutectic solvents (DES) and developed two simple and robust mathematical models to predict the use of solvents with the most suitable physicochemical properties of their components for the LPMO biocatalytic reaction. Easily accessible, non-toxic, and relatively inexpensive components were selected to create the deep eutectic solvents according to the principles of green chemistry and sustainable development. LPMO activity was initially spectrophotometrically tested in NaAc buffer pH 6,4 for comparison. Then, enzyme activity was tested in prepared solvents using the same method with buffer as a blank. Furthermore, DES were mathematically described using the COSMO-RS software package. The obtained values were grouped into 10 intervals to create a sigma profile of the solvents. Based on these intervals, multiple linear regression and piecewise linear regression models were developed using the Statistica 13.0 software package.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:690731
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-04-02 09:00:13