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master's thesis
Funkcije astrocita u fragilnom X sindromu: in vitro istraživanje
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:296994
Begić, Darija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Begić, D. (2022). Funkcije astrocita u fragilnom X sindromu: in vitro istraživanje (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:296994

Begić, Darija. "Funkcije astrocita u fragilnom X sindromu: in vitro istraživanje." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:296994

Begić, Darija. "Funkcije astrocita u fragilnom X sindromu: in vitro istraživanje." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:296994

Begić, D. (2022). 'Funkcije astrocita u fragilnom X sindromu: in vitro istraživanje', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 27 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:296994

Begić D. Funkcije astrocita u fragilnom X sindromu: in vitro istraživanje [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 December 27] Available at: https://urn.nsk.hr/urn:nbn:hr:159:296994

D. Begić, "Funkcije astrocita u fragilnom X sindromu: in vitro istraživanje", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:296994

Metadata
TitleFunkcije astrocita u fragilnom X sindromu: in vitro istraživanje
Title (english)Astroglial function in fragile X syndrome: an in vito investigation
AuthorDarija Begić
MentorVladimir Mrša (mentor)
Committee memberVišnja Besendorfer (predsjednik povjerenstva)
Committee memberDragomira Majhen (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2022-07-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Fragilni X sindrom (FXS) vodeći je monogenski uzrok poremećaja iz spektra autizma (ASD). Uzrokovan je gubitkom ekspresije FMRP proteina zbog ekspanzije CGG-a. Iako se većina studija usredotočila na uloge FMRP-a u neuronima zbog kognitivnih nedostataka u FXS-u, također se pokazalo da gubitak FMRP-a u astrocitima doprinosi patogenezi FXS-a. Cilj ovog projekta je utvrditi funkcije FMRP-a u astrocitima razvojem strategije prekomjerne ekspresije FMRP-a u astrocitima. U tu svrhu, bila je potrebna karakterizacija astrocita i razvoj učinkovite metode transfekcije. Posebna je pažnja posvećena purinergičkom odgovoru s fokusom na purinergički receptor P2X7. Pokazali smo pojačanu regulaciju purinergičkog odgovora Fmr1 KO astrocita, posebno u pogledu lučenja interleukina-6 (IL-6) nakon tretmana ATP-om. Najučinkovitija i najfleksibilnija metoda transfekcije je magnetofekcija.
Abstract (english)
Fragile X syndrome (FXS) is the leading monogenic cause of autism spectrum disorders (ASD). It is caused by the CGG expansion-induced loss of fragile X mental retardation protein (FMRP) expression. Although most studies focused on the roles of FMRP in neurons due to cognitive deficits in FXS, the loss of FMRP in astroglia has also been shown to contribute to FXS pathogenesis. The aim of this project is to determine the astrocytic functions of FMRP by developing a strategy to overexpress FMRP in astrocytes. To access that, astrocyte characterization and development of an efficient transfection assay were needed. Particular attention was paid to the purinergic response with a focus on the P2X7 purinergic receptor between the C8-D1A cell line and primary astrocytic cell culture (APC). We demonstrated upregulation of the purinergic response of Fmr1 KO astrocytes especially regarding interleukin-6 (IL-6) secretion following ATP treatment. The most efficient and flexible transfection method is magnetofection.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:296994
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-04-08 10:33:16