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undergraduate thesis
Izolacija plazmida pBAD-HheC, pUC-GW-RadH i pUC-GW-NoxV iz velikog volumena kulture bakterije Escherichia coli
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:045114
Vasung, Emma Karolina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Vasung, E. K. (2022). Izolacija plazmida pBAD-HheC, pUC-GW-RadH i pUC-GW-NoxV iz velikog volumena kulture bakterije Escherichia coli (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:045114

Vasung, Emma Karolina. "Izolacija plazmida pBAD-HheC, pUC-GW-RadH i pUC-GW-NoxV iz velikog volumena kulture bakterije Escherichia coli." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:045114

Vasung, Emma Karolina. "Izolacija plazmida pBAD-HheC, pUC-GW-RadH i pUC-GW-NoxV iz velikog volumena kulture bakterije Escherichia coli." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:045114

Vasung, E. K. (2022). 'Izolacija plazmida pBAD-HheC, pUC-GW-RadH i pUC-GW-NoxV iz velikog volumena kulture bakterije Escherichia coli', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 15 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:045114

Vasung EK. Izolacija plazmida pBAD-HheC, pUC-GW-RadH i pUC-GW-NoxV iz velikog volumena kulture bakterije Escherichia coli [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 July 15] Available at: https://urn.nsk.hr/urn:nbn:hr:159:045114

E. K. Vasung, "Izolacija plazmida pBAD-HheC, pUC-GW-RadH i pUC-GW-NoxV iz velikog volumena kulture bakterije Escherichia coli", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:045114

Metadata
TitleIzolacija plazmida pBAD-HheC, pUC-GW-RadH i pUC-GW-NoxV iz velikog volumena kulture bakterije Escherichia coli
Title (english)Isolation of plasmids pBAD-HheC, pUC-GW-RadH and pUC-GW-NoxV from a large volume of Escherichia coli culture
AuthorEmma Karolina Vasung
MentorMarina Svetec Miklenić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2022-09-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Plazmidi su iznimno važni u genetičkom inženjerstvu, gdje se upotrebljavaju kao vektori, tj. molekule DNA koje omogućuju umnožavanje kloniranog fragmenta DNA u stanici domaćinu. U genetičkom inženjerstvu E. coli se koristi kao univerzalan domaćin za kloniranje. Cilj ovog rada bio je izolirati plazmide pBAD-HheC, pUC-GW-RadH i pUC-GW-NoxV iz velikog volumena bakterije Escherichia coli. Nakon izolacije i pročišćavanja plazmidne DNA, provedena je gel-elektroforeza radi provjere uspješnosti izolacije. Stvarna struktura izoliranih plazmida provjerena je restrikcijskom analizom pomoću dva restrikcijska enzima. Veličina dobivenih fragmenata nakon cijepanja bila je u skladu s restrikcijskim mapama plazmida.
Abstract (english)
Plasmids play an important role in genetic engineering; they are used as vectors, i.e., DNA molecules which allow the cloned DNA fragment to be propagated in the host cell. In genetic engineering, E. coli is used as a universal host for cloning. The aim of this work was to isolate the plasmids pBAD-HheC, pUC-GW-RadH and pUC-GW-NoxV from a large volume of E. coli culture. Following the isolation and purification of the plasmid DNA, gel-electrophoresis was used to determine whether the DNA isolation was successful. The actual structure of the isolated plasmids was confirmed using restriction enzyme analysis. The size of the cleaved fragments matched the restriction maps of the plasmids.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:045114
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-04-09 12:42:41