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undergraduate thesis
Rast CHO stanica u bazalnom hranjivom mediju s proteinskim hidrolizatom sjemenki lana i albuminom
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:432717
Galić, Mia
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Galić, M. (2022). Rast CHO stanica u bazalnom hranjivom mediju s proteinskim hidrolizatom sjemenki lana i albuminom (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:432717

Galić, Mia. "Rast CHO stanica u bazalnom hranjivom mediju s proteinskim hidrolizatom sjemenki lana i albuminom." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:432717

Galić, Mia. "Rast CHO stanica u bazalnom hranjivom mediju s proteinskim hidrolizatom sjemenki lana i albuminom." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:432717

Galić, M. (2022). 'Rast CHO stanica u bazalnom hranjivom mediju s proteinskim hidrolizatom sjemenki lana i albuminom', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 07 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:432717

Galić M. Rast CHO stanica u bazalnom hranjivom mediju s proteinskim hidrolizatom sjemenki lana i albuminom [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 May 07] Available at: https://urn.nsk.hr/urn:nbn:hr:159:432717

M. Galić, "Rast CHO stanica u bazalnom hranjivom mediju s proteinskim hidrolizatom sjemenki lana i albuminom", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:432717

Metadata
TitleRast CHO stanica u bazalnom hranjivom mediju s proteinskim hidrolizatom sjemenki lana i albuminom
Title (english)CHO cell growth in basal nutrient medium with flaxseed protein hydrolysate and albumin
AuthorMia Galić
MentorIgor Slivac (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2022-06-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U ovom radu obrađena je tema uzgoja adherentnih stanica ovarija kineskog hrčka CHO DP- 12 u bazalnom hranjivom mediju (DMEM) sa sljedećim dodacima: 10% fetalnog goveđeg seruma (standard), s dodatkom albumina i hidrolizata proteina uljne pogače lana te s dodatkom samog albumina. Albumin je glavni protein u fetalnom goveđem serumu te se ispituje njegov značaj za rast i održavanje stanica, dok se biljni proteinski hidrolizati često dodaju kao zamjena proteinima životinjskog podrijetla. Krajnji cilj ovakvih ispitivanja jest pronaći optimalan medij za uzgoj stanica sa što manjom količinom dodanog seruma, budući da postoji mnogo problema vezanih za upotrebu seruma kao što su cijena, sigurnost, potencijalne kontaminacije te etička pitanja vezana za proizvodnju seruma. Eksperiment je proveden na način da su stanice uzgajane u navedenim medijima te je određenih dana bojilom Trypan plavo praćen rast stanica. Rezultati pokazuju da je najveća koncentracija i najbrži rast u mediju koji sadrži 10% fetalnog goveđeg seruma, a najmanja koncentracija u mediju koji je sadržavao samo albumin. Albumin sa ili bez dodanih lanenih hidrolizata nije jednakovrijedna zamjena za fetalni serum.
Abstract (english)
The subject of this thesis is culturing adherent chinese hamster ovary cell line CHO DP-12 in basal culture media (DMEM) containing different supplements: 10% fetal bovine serum (standard), with different concentrations of albumin and protein hydrolysates and with the addition of albumin itself. Albumin is the major protein in fetal bovine serum and its effect on cell growth and maintenance is examined in order to find the optimal medium for culturing serum-free cells, as there are many problems regarding serum use such as price, safety, potential contamination, and ethical issues related to serum production. The experiment was carried out in such a way that the cells were grown in the previously mentioned media, and on certain days the cells were counted using the Trypan blue method, thus monitoring their growth and development. The results show that the highest concentration and the fastest growth is in the medium containing 10% of fetal bovine serum, and the lowest concentration in the medium containing only albumin. Supplementation of DMEM with albumin and/or flaxseed hydrolysates cannot is not recommended as serum replacement.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:432717
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-04-10 12:08:09