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undergraduate thesis
Priprema elektrokompetentnih stanica bakterije Escherichia coli MC1061
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:183147
Ajduković, Karla
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Ajduković, K. (2022). Priprema elektrokompetentnih stanica bakterije Escherichia coli MC1061 (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:183147

Ajduković, Karla. "Priprema elektrokompetentnih stanica bakterije Escherichia coli MC1061." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:183147

Ajduković, Karla. "Priprema elektrokompetentnih stanica bakterije Escherichia coli MC1061." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:183147

Ajduković, K. (2022). 'Priprema elektrokompetentnih stanica bakterije Escherichia coli MC1061', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 15 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:183147

Ajduković K. Priprema elektrokompetentnih stanica bakterije Escherichia coli MC1061 [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 July 15] Available at: https://urn.nsk.hr/urn:nbn:hr:159:183147

K. Ajduković, "Priprema elektrokompetentnih stanica bakterije Escherichia coli MC1061", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:183147

Metadata
TitlePriprema elektrokompetentnih stanica bakterije Escherichia coli MC1061
Title (english)Preparation of electrocompetent cells of the bacterium Escherichia coli
AuthorKarla Ajduković
MentorMarina Svetec Miklenić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2022-09-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Genetička transformacija jest ulazak strane DNA u stanicu iz njene neposredne okoline. Razlikujemo više metoda transformacije poput kemijske metode, biolističke, transformacije pomoću ultrazvuka (sonoporacije), „tribos“ transformacije i elektrotransformacije (elektroporacije). U ovom radu detaljnije je opisana metoda elektroporacije, transformacija koja se temelji na primjeni kratkog pulsa visokog napona. Elektroporacija je jedna od najučinkovitijih metoda transformacije kod bakterija Escherichia coli. Cilj ovog rada bio je pripremiti elektrokompetentne stanice bakterije Escherichia coli soja MC1061 te provjeriti kompetentnost pripremljenih stanica transformacijom s plazmidom pLS42.
Abstract (english)
Genetic transformation involves the entry of foreign DNA into the cell. We distinguish several transformation methods such as chemical method, biolistic method, sonic transformation (sonoporation), “tribos” transformation and electrotransformation (electroporation). Electrotransformation, transformation based on the application of a short puls of high voltage is described in more detail in this work. Electroporation is one of the most effective transformation methods when it comes to Escherichia coli. The aim of this study was to prepare electrocompetent cells of Escherichia coli strain MC1061 and to check the competence of the prepared cells by transformation with plasmid pLS42.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:183147
Study programmeTitle: Nutrition
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-04-11 08:52:11