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undergraduate thesis
Konstrukcija plazmida pBAD-NoxV
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:183781
Šubar, Karla
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Šubar, K. (2022). Konstrukcija plazmida pBAD-NoxV (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:183781

Šubar, Karla. "Konstrukcija plazmida pBAD-NoxV." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:183781

Šubar, Karla. "Konstrukcija plazmida pBAD-NoxV." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:183781

Šubar, K. (2022). 'Konstrukcija plazmida pBAD-NoxV', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:183781

Šubar K. Konstrukcija plazmida pBAD-NoxV [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2024 December 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:183781

K. Šubar, "Konstrukcija plazmida pBAD-NoxV", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:183781

Metadata
TitleKonstrukcija plazmida pBAD-NoxV
Title (english)Construction of plasmid pBAD- NoxV
AuthorKarla Šubar
MentorMarina Svetec Miklenić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2022-09-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Tehnologija rekombinantne DNA je skup molekularno - genetičkih metoda koje omogućuju uvođenje preciznih genetičkih promjena u živu stanicu ili organizam u svrhu konstrukcije rekombinantnih molekula DNA. Primjena ove tehnologije u biologiji i srodnim znanostima rezultirala je mnogim revolucionarnim otkrićima. U ovom radu spomenutim metodama konstruiran je plazmid pBAD – NoxV. Plazmid je konstruiran ugradnjom fragmenta (inserta) DNA izdvojenog iz plazmida pUC – GW – NoxV, koji sadrži gen NoxV Ecoli Opti, u okosnicu plazmida pBADHHEC koji je korišten kao vektor te sadrži gene za rezistenciju na antibiotik ampicilin, ishodište replikacije u bakteriji Escherichii coli, gen araC i promotor araBAD. Iz odabranih transformanata bakterije E. coli izolirana je plazmidna DNA čija je struktura provjerena restrikcijskom analizom. Rezultati analize su potvrdili uspješnu konstrukciju plazmida pBAD - NoxV.
Abstract (english)
Recombinant DNA technology is a set of molecular-genetic methods that enable precise genetic changes to be introduced into a cell or organism for the purpose of constructing recombinant DNA molecules. The application of this technology in biology and related sciences has resulted in many revolutionary discoveries. In this work, the plasmid pBAD – NoxV was constructed using by mentioned methods. The plasmid was constructed by incorporating a fragment (insert) of DNA isolated from the plasmid pUC - GW - NoxV containing the NoxV Ecoli opti gene into the backbone of the plasmid pBADHHEC, which was used as a vector and contains genes for resistance to the antibiotic ampicillin, the origin of replication in the Escherichia coli bacterium, the araC gene and the araBAD promoter. From the selected transformants, plasmid DNA was isolated and subjected to restriction analysis. The result of the analysis confirmed the successful construction of the plasmid pBAD - NoxV.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:183781
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-04-15 10:32:06