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undergraduate thesis
Bakterijski izolati iz roda Pseudomonas kao inhibitori rasta micelija slatkovodnih patogenih mikroorganizama iz skupine Oomycota
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:413069
Majerčak-Škorlić, Matilda
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Majerčak Škorlić, M. (2023). Bakterijski izolati iz roda Pseudomonas kao inhibitori rasta micelija slatkovodnih patogenih mikroorganizama iz skupine Oomycota (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:413069

Majerčak Škorlić, Matilda. "Bakterijski izolati iz roda Pseudomonas kao inhibitori rasta micelija slatkovodnih patogenih mikroorganizama iz skupine Oomycota." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:413069

Majerčak Škorlić, Matilda. "Bakterijski izolati iz roda Pseudomonas kao inhibitori rasta micelija slatkovodnih patogenih mikroorganizama iz skupine Oomycota." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:413069

Majerčak Škorlić, M. (2023). 'Bakterijski izolati iz roda Pseudomonas kao inhibitori rasta micelija slatkovodnih patogenih mikroorganizama iz skupine Oomycota', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:413069

Majerčak Škorlić M. Bakterijski izolati iz roda Pseudomonas kao inhibitori rasta micelija slatkovodnih patogenih mikroorganizama iz skupine Oomycota [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:159:413069

M. Majerčak Škorlić, "Bakterijski izolati iz roda Pseudomonas kao inhibitori rasta micelija slatkovodnih patogenih mikroorganizama iz skupine Oomycota", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:413069

Metadata
TitleBakterijski izolati iz roda Pseudomonas kao inhibitori rasta micelija slatkovodnih patogenih mikroorganizama iz skupine Oomycota
Title (english)Bacterial isolates from the genus Pseudomonas as inhibitors of mycelial growth of freshwater oomycete pathogens
AuthorMatilda Majerčak-Škorlić
MentorAna Bielen (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2023-06-15, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Patogeni mikroorganizmi Aphanomyces astaci i Saprolegnia parasitica uzrokuju gubitke u slatkovodnoj akvakulturi rakova i riba te je potrebno razviti ekološki prihvatljive metode njihove kontrole. Cilj ovog rada bio je istražiti inhibicijski potencijal 25 izolata roda Pseudomonas prema rastu micelija ovih patogena. Najprije je filogenetskom analizom gena koji kodira za 16S rRNA izvršena identifikacija izolata, i to 7/25 izolata (28 %) do vrste, 4/25 izolata (16 %) do podgrupe te 11/25 (44 %) do grupe unutar roda Pseudomonas. Nadalje, in vitro testovima inhibicije je utvrđeno da je čak 44 % izolata snažno inhibiralo rast micelija A. astaci te su se najjačima istaknuli izolati grupe fluorescens. Inhibicija patogena S. parasitica bila je znatno slabija, ali ipak primjetna kod nekih izolata iz grupa fluorescens i lutea. Zaključno, rezultati ovog rada ukazuju na moguću primjenu bakterija iz roda Pseudomonas u svrhu biokontrole patogena A. astaci i S. parasitica u akvakulturi.
Abstract (english)
The pathogenic microorganisms Aphanomyces astaci and Saprolegnia parasitica cause losses in freshwater aquaculture and it is necessary to develop environmentally acceptable methods of their control. The aim of this study was to investigate the inhibitory potential of 25 isolates of the genus Pseudomonas against their mycelial growth. First, the phylogenetic analysis of the gene that encodes 16S rRNA was applied to identify the isolates, namely 7/25 isolates (28%) were classified to the species level, 4/25 isolates (16%) to the subgroup, and 11/25 (44%) to the group within the genus Pseudomonas. Furthermore, in vitro inhibition tests showed that 44% of the isolates strongly inhibited the growth of A. astaci mycelia, mostly the isolates from the fluorescens group. Inhibition of the pathogen S. parasitica was significantly weaker but still noticeable for some isolates from the fluorescens and lutea groups. In conclusion, the results of this work indicate the possible use of bacteria of the genus Pseudomonas for A. astaci and S. parasitica biocontrol in aquaculture.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:413069
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-04-19 11:31:44