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undergraduate thesis
Morfološka i fenotipska karakterizacija ljubičastih nesumpornih bakterija (Rhodobacter capsulatus i Rhodobacter azotoformans)
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:272532
Zrile, Antonija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for General Microbiology and Food Microbiology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Zrile, A. (2023). Morfološka i fenotipska karakterizacija ljubičastih nesumpornih bakterija (Rhodobacter capsulatus i Rhodobacter azotoformans) (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:272532

Zrile, Antonija. "Morfološka i fenotipska karakterizacija ljubičastih nesumpornih bakterija (Rhodobacter capsulatus i Rhodobacter azotoformans)." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:272532

Zrile, Antonija. "Morfološka i fenotipska karakterizacija ljubičastih nesumpornih bakterija (Rhodobacter capsulatus i Rhodobacter azotoformans)." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:272532

Zrile, A. (2023). 'Morfološka i fenotipska karakterizacija ljubičastih nesumpornih bakterija (Rhodobacter capsulatus i Rhodobacter azotoformans)', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:272532

Zrile A. Morfološka i fenotipska karakterizacija ljubičastih nesumpornih bakterija (Rhodobacter capsulatus i Rhodobacter azotoformans) [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 November 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:272532

A. Zrile, "Morfološka i fenotipska karakterizacija ljubičastih nesumpornih bakterija (Rhodobacter capsulatus i Rhodobacter azotoformans)", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:272532

Metadata
TitleMorfološka i fenotipska karakterizacija ljubičastih nesumpornih bakterija (Rhodobacter capsulatus i Rhodobacter azotoformans)
Title (english)Morphological and phenotypic characterization of purple non-sulfur bacteria (Rhodobacter capsulatus and Rhodobacter azotoformans)
AuthorAntonija Zrile
MentorIva Čanak (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for General Microbiology and Food Microbiology)
Zagreb
Defense date and country2023-09-08, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Ljubičaste nesumporne bakterije su fotosintetski prokarioti koji svjetlosnu energiju pretvaraju u kemijsku procesom anoksigene fotosinteze. Najčešće se izoliraju iz otpadnih voda i ekstremnih staništa. Njihova prisutnost dovodi do biorazgradnje organskih i anorganskih spojeva, kao i do biosinteze visokovrijednih nusprodukata. Biomasa ljubičastih nesumpornih bakterija sadrži visoku koncentraciju proteina, esencijalnih aminokiselina, pigmenata i vitamina. Najvažnija primjena ove skupine bakterija je u obradi otpadnih voda prehrambene industrije te u biotehnološkoj proizvodnji biopolimera. U ovom radu su određene morfološke i fenotipske karakteristike dviju vrsta ljubičastih nesumpornih bakterija-Rhodobacter capsulatus JCM 21090 i Rhodobacter azotoformans JCM 9340. Rezultati istraživanja pokazali su da se radi o gram-negativnim, pokretnim bakterijama, koje prilikom rasta na krutoj hranjivoj podlozi stvaraju glatke okrugle kolonije. Tijekom duljeg uzgoja u tekućoj hranjivoj podlozi došlo je do sinteze pigmenta i obojenja podloge u crvenu boju. Fenotipskom identifikacijom koristeći SDS elektroforezu, dobiven je prikaz ukupnih i površinskih proteina, koji se međusobno razlikuju ovisno o periodu inkubacije bakterija. Dobiveni rezultati daju važan doprinos za buduće karakterizacije i primjenu ljubičastih nesumpornih bakterija.
Abstract (english)
Purple non-sulfur bacteria are photosynthetic prokaryotes that convert light energy into the chemical energy through the process of anoxygenic photosynthesis. They are most often isolated from wastewater and extreme habitats. Their presence leads to the biodegradation of organic and inorganic compounds, as well as to the biosynthesis of high-value byproducts. The biomass of purple non-sulfur bacteria contains a high concentration of proteins, essential amino acids, pigments and vitamins. The most important use of this type of bacteria is in the treatment of wastewater from the food industry and in the biotechnological production of biopolymers. In this research, the morphological and phenotypic characteristics were determined of two strains of purple non-sulfur bacteria-Rhodobacter capsulatus JCM 21090 and Rhodobacter azotoformans JCM 9340. The results of the research showed that they are gram-negative, motile bacteria which create smooth round colonies when growing on a solid nutrient substrate. During the long cultivation in the liquid nutrient medium, pigment synthesis occurred and the medium was colored red. By phenotypic identification using SDS electrophoresis, a display of total and surface proteins was obtained, which differ from each other depending on the period of bacterial incubation. The obtained results provide an important contribution to the future characterization and application of purple non-sulfur bacteria.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:272532
Study programmeTitle: Food Technology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-04-19 12:39:02