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undergraduate thesis
Primjena "Layer by Layer" nanoinkapsulacije Limosilactobacillius sojeva za dostavu probiotičkih stanica in situ
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:344932
Vitaliani, Lucija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Vitaliani, L. (2023). Primjena "Layer by Layer" nanoinkapsulacije Limosilactobacillius sojeva za dostavu probiotičkih stanica in situ (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:344932

Vitaliani, Lucija. "Primjena "Layer by Layer" nanoinkapsulacije Limosilactobacillius sojeva za dostavu probiotičkih stanica in situ." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:344932

Vitaliani, Lucija. "Primjena "Layer by Layer" nanoinkapsulacije Limosilactobacillius sojeva za dostavu probiotičkih stanica in situ." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:344932

Vitaliani, L. (2023). 'Primjena "Layer by Layer" nanoinkapsulacije Limosilactobacillius sojeva za dostavu probiotičkih stanica in situ', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 05 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:344932

Vitaliani L. Primjena "Layer by Layer" nanoinkapsulacije Limosilactobacillius sojeva za dostavu probiotičkih stanica in situ [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 November 05] Available at: https://urn.nsk.hr/urn:nbn:hr:159:344932

L. Vitaliani, "Primjena "Layer by Layer" nanoinkapsulacije Limosilactobacillius sojeva za dostavu probiotičkih stanica in situ", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:344932

Metadata
TitlePrimjena "Layer by Layer" nanoinkapsulacije Limosilactobacillius sojeva za dostavu probiotičkih stanica in situ
Title (english)Application of "LbL" nanoencapsulation for enhanced delivery of Limosilactobacillus probiotic cells in situ
AuthorLucija Vitaliani
MentorJasna Novak (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2023-09-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Nanoinkapsulacija je suvremeni pristup za ciljanu zaštitu i kontroliranu dostavu probiotičkih sojeva ili pripadajućih postbiotika odnosno bioaktivnih spojeva in situ. Upravo cilj ovog rada je definiranje zaštitnog učinka „layer by layer“ (LbL) nanoinkapsulacije bakterijskih stanica Limosilactobacillus sojeva analizom postotka preživljavanja suhog aktivnog pripravka, neposredno nakon liofilizacije, te mjesec dana tijekom pohrane za pojačanu biodostupnost. Provedena je i nanoinkapsulacija egzopolisharida ovih sojeva koji se istražuju kao postbiotici. Učinkovito formiranje nanokapsula bakterijskih stanica Limosilactobacillus fermentum potvrđeno je mjerenjem zeta potencijala, temeljem kojeg je zabilježena promjena iz pozitivnog u negativni naboj, i obrnuto, tijekom faza nanošenja slojeva elektrolita metodom „sloj po sloj“ nanoinkapsulacije. Postotak preživljavanja nanoinkapsuliranih sojeva Lb. fermentum veći je nakon inkubacije u samom želučanom soku naspram postotka preživljavanja intaktnih sojeva nakon inkubacije u želučanom soku i soku tankog crijeva što upućuje na zaštitnu ulogu „LbL“ nanoinkapsulacije i mogućnost pojačanje biodostupnosti in situ.
Abstract (english)
Nanoencapsulation is a modern approach for targeted protection and controlled in situ delivery of probiotic strains or associated postbiotics or bioactive compounds. The aim of this work is to define the protective effect of "layer by layer" (LbL) nanoencapsulation of bacterial cells of Limosilactobacillus strains by analyzing the survival percentage of the active freeze-dried cells, immediately after lyophilization, and after one month during storage to enhance bioavailability. Nanoencapsulation of the exopolysaccharides of these strains, which are being investigated as postbiotics, was also carried out. The effective formation of nanocapsules of Limosilactobacillus fermentum bacterial cells was confirmed by measuring the zeta potential, based on which the change from positive to negative charge and vice versa was recorded during the stages of layering of electrolyte layers using the "LbL" nanoencapsulation. Survival percentage of nanoencapsulated strains of Lb. fermentum is higher after incubation in gastric juice compared to the survival percentage of intact strains after incubation in gastric juice and small intestine juice, which points to the protective role of "LbL" nanoencapsulation and the possibility of enhancing bioavailability in situ.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:344932
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-04-26 08:46:03