prikaz prve stranice dokumenta The role of autophagy in iron metabolism: in vivo and in vitro studies
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master's thesis
The role of autophagy in iron metabolism: in vivo and in vitro studies
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. urn:nbn:hr:159:158915
Knežević, Tea
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Knežević, T. (2022). The role of autophagy in iron metabolism: in vivo and in vitro studies (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:158915

Knežević, Tea. "The role of autophagy in iron metabolism: in vivo and in vitro studies." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:158915

Knežević, Tea. "The role of autophagy in iron metabolism: in vivo and in vitro studies." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2022. https://urn.nsk.hr/urn:nbn:hr:159:158915

Knežević, T. (2022). 'The role of autophagy in iron metabolism: in vivo and in vitro studies', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:158915

Knežević T. The role of autophagy in iron metabolism: in vivo and in vitro studies [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2022 [cited 2025 February 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:158915

T. Knežević, "The role of autophagy in iron metabolism: in vivo and in vitro studies", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2022. Available at: https://urn.nsk.hr/urn:nbn:hr:159:158915

Metadata
TitleThe role of autophagy in iron metabolism: in vivo and in vitro studies
Title (croatian)Uloga autofagije u metabolizmu željeza: in vivo i in vitro istraživanja
AuthorTea Knežević
MentorVladimir Mrša (mentor)
Committee memberVladimir Mrša (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2022-07-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Iron is one of the most essential elements in our body, as it is required in processes like erythropoiesis, cellular respiration, DNA synthesis, proliferation, host defence and cell signalling. The lack of iron causes various diseases, the most widespread being amenia. Imbalance in iron homeostasis results from dysregulated expression of proteins connected to iron metabolism, the most important being the hepcidin, regulatory hormone and the ferroportin, iron exporter. A recent study showed an important role of autophagy in the regulation of iron metabolism. Autophagy is a process of degradation in macrophages, which has a role in tissue homeostasis and inflammatory response in macrophages. In this project we continued in vivo studies by using mice deficient for autophagy in macrophages (LysM-Atg5-/-) aged 5 to 19 weeks, and in vitro studies by using primary macrophage culture. We observed that erythrophagocytosis strongly induces autophagy in cultured macrophages and that deficiency in autophagy in LysM-Atg5-/- mice leads to progressive decreased of iron in the spleen and iron overload in liver. The results confirm that autophagy plays a crucial role in maintaining iron homeostasis.
Abstract (french)
Le fer est l'un des éléments les plus essentiels de notre organisme, car il est nécessaire dans des processus tels que l'érythropoïèse, la respiration cellulaire, la synthèse de l’ADN, la prolifération, la défense de l'hôte et la signalisation cellulaire. Le manque de fer est à l'origine de diverses maladies, la plus répandue étant l’anémie. Le déséquilibre de l'homéostasie du fer résulte d’une dérégulation de l'expression des protéines liées au métabolisme du fer, les plus importantes étant l'hepcidine, hormone de régulation, et la ferroportine, exportateur de fer. Une étude récente a montré le rôle important de l’autophagie dans la régulation du métabolisme du fer. L’autophagie étant un processus de dégradation qui a un rôle dans l’homéostasie tissulaire et la réponse inflammatoire dans les macrophages. Dans ce projet, nous avons mené des études in vivo en utilisant les souris déficientes pour l’autophagie dans les macrophages (LysM-Atg5-/-) âgées de 5 à 19 semaines, et des études in vitro en utilisant des cultures de macrophages primaires. Nous avons observé que l’érythropoïèse induit fortement l’autophagie dans les macrophages en culture et que le déficit d’autophagie chez les souris LysM-Atg5-/- entraîne une diminution progressive du fer dans la rate et une surcharge en fer dans le foie. Ces résultats confirment que l'autophagie joue un rôle crucial dans le maintien de l’homéostasie du fer.
Keywords
Keywords (french)
Languageenglish
URN:NBNurn:nbn:hr:159:158915
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2024-04-29 09:38:10