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undergraduate thesis
Optimizacija parametara uzgoja Euglene gracilis primjenom metode odzivnih površina
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:872811
Jukić, Ivan
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Jukić, I. (2014). Optimizacija parametara uzgoja Euglene gracilis primjenom metode odzivnih površina (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:872811

Jukić, Ivan. "Optimizacija parametara uzgoja Euglene gracilis primjenom metode odzivnih površina." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:872811

Jukić, Ivan. "Optimizacija parametara uzgoja Euglene gracilis primjenom metode odzivnih površina." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:872811

Jukić, I. (2014). 'Optimizacija parametara uzgoja Euglene gracilis primjenom metode odzivnih površina', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 16 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:872811

Jukić I. Optimizacija parametara uzgoja Euglene gracilis primjenom metode odzivnih površina [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2025 January 16] Available at: https://urn.nsk.hr/urn:nbn:hr:159:872811

I. Jukić, "Optimizacija parametara uzgoja Euglene gracilis primjenom metode odzivnih površina", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:872811

Metadata
TitleOptimizacija parametara uzgoja Euglene gracilis primjenom metode odzivnih površina
Title (english)Optimization of Euglena gracilis cultivation parameters by response surface methodology
AuthorIvan Jukić
MentorTonči Rezić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2014-09-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U budućnosti očekuje se energetska kriza zbog ograničenih fosilnih izvora energije. Jedna od strategija je nadoknaditi dio kapljevitih goriva proizvodnjom biodizela iz lipida mikoralgi. Istraživanje se temelji na proizvodnji lipida iz alge Euglena gracilis te je izveden foto-miksotrofni uzgoj u foto–bioreaktoru s miješalom. Tijekom ovog istraživanja, sastav hranjive podloge i procesni parametri za foto-heterotrofni uzgoj su optimizirani kako bi se definirali adekvatni uvjetiza uzgoj biomase mikroalgi i proizvodnju lipida. Zbog niske cijene i dostupnog izvora ugljika i dušika, kukurzna močevina (CSL) je korištena za rast Euglene gracilis i proizvodnju lipida. Optimizacijom sastava hranjive podloge i uvjeta uzgoja, te korištenjem metode odzivnih površina (RSM) povećana je proizvodnja lipida i biomase do 29 % suhe tvari biomase postupkom u dva stupnja uzgoja u jednom foto–bioreaktoru. Određene optimalne vrijednosti procesenih parametara (koncentracija kukuruzne močevine (CSL 26,4-26,6 g/L ),brzina miješanja (rpm199-283 min-1), aeracija(0,49-0,57 v/v min)) koristiti će se u daljnem istraživanju dobivanja lipida iz biomase mikroalge E. gracilis.
Abstract (english)
In the future, due to limited fossil energy resources, a crisis of energy,is expected. One strategy is to compensate a part of the liquid fuel production of biodiesel from microalgal lipids. The studyis based on the production of lipids from microalgae Euglena gracilis and it was performedphoto-mixotrophic cultivation in the stirred tank photo-bioreactor. During this research, medium composition and operational conditions of photo-bioreactor were optimized in order to define adequate cultivation conditions for algae biomass and lipid production. As low cost and available complex carbon nitrogen source, corn steep liquor was used to promote Euglena gracilis growth and lipid production. Due to optimization of medium composition and cultivation conditions,lipid and biomass production was increased up to 29 % in a two stage cultivation process inside one photo-bioreactor. Thedetermined optimal values of the process parameters (corn steep liquor (CSL 26,4-26,6g/L ), steering speed (rpm 199 -283 min-1), aeration(0,49-0,57 v/v min)) will be used in the following study to obtain lipids from E. gracilis microalgae biomass.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:872811
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2017-04-27 11:43:29