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master's thesis
Role of GPR109A in psoriasis
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:730726
Leko, Lucija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Leko, L. (2023). Role of GPR109A in psoriasis (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:730726

Leko, Lucija. "Role of GPR109A in psoriasis." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:730726

Leko, Lucija. "Role of GPR109A in psoriasis." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:730726

Leko, L. (2023). 'Role of GPR109A in psoriasis', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 17 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:730726

Leko L. Role of GPR109A in psoriasis [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2025 February 17] Available at: https://urn.nsk.hr/urn:nbn:hr:159:730726

L. Leko, "Role of GPR109A in psoriasis", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:730726

Metadata
TitleRole of GPR109A in psoriasis
Title (croatian)Uloga GPR109A receptora u psorijazi
AuthorLucija Leko
MentorIgor Stuparević (mentor)
Committee memberVišnja Besendorfer (predsjednik povjerenstva)
Committee memberKristina Radošević (član povjerenstva)
Committee memberIgor Stuparević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2023-07-03, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Psoriasis is a chronic, inflammatory skin disease characterized by keratinocyte hyperproliferation and immune cell infiltration. In this study, bioinformatic analysis showed that HCAR2, human gene encoding a short-chain fatty acid receptor GPR109A, was expressed in keratinocytes and macrophages in lesioned skin of psoriatic patients. In imiquimod (IMQ) induced mouse model immunostaining showed GPR109A to be located in keratinocytes and dermal inflammatory cells. We showed that Hcar2 expression was affected in IMQ-induced mouse model and that mice lacking GPR109A had an aggravated psoriasis-like phenotype. Levels of inflammatory mediators like MPO, LCN2 and IL-1β were elevated along with Il-17a expression, all indicating GPR109A has a protective role in psoriasis. Further research should focus on using GPR109A agonists, such as butyrate, as potential therapeutics in treatment of psoriasis.
Abstract (croatian)
Psorijaza je kronična, upalna, kožna bolest karakterizirana hiperproliferacijom keratinocita i infiltracijom stanica imunološkog sustava u kožu. U ovom istraživanju, bioinformatička analiza je pokazala da je HCAR2, ljudski gen koji kodira za receptor kratkolančanih masnih kiselina GPR109A, izražen u keratinocitima i makrofagima u oštećenoj koži pacijenata s psorijazom. Imunohistokemijsko bojanje u mišjem imikvimod (IMQ) tretiranom modelu pokazalo je da je GPR109A prisutan u keratinocitima i infiltracijskim stanicama imunološkog sustava dermisa. Pokazali smo da je ekspresija gena Hcar2 izmjenjena u mišjem IMQ tretiranom modelu te da miševi koji nemaju GPR109A imaju pogoršan psorijatični fenotip. Razine upalnih medijatora poput MPO, LCN2 i IL-1β su povišene zajedno s ekspresijom Il-17a, što sve ukazuje na zaštitnu ulogu GPR109A u psorijazi. Daljnja istraživanja trebala bi se usredotočiti na upotrebu agonista GPR109A, poput butirata, kao potencijalne terapije u liječenju psorijaze.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:159:730726
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0) icon
Created on2024-04-29 10:57:06