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undergraduate thesis
Obilježavanje S-adenozil-metionin sintetaze hemaglutininskom-oznakom i njezina detekcija na površini stanične stijenke kvasca
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:292823
Vukšić, Anđela
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Vukšić, A. (2023). Obilježavanje S-adenozil-metionin sintetaze hemaglutininskom-oznakom i njezina detekcija na površini stanične stijenke kvasca (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:292823

Vukšić, Anđela. "Obilježavanje S-adenozil-metionin sintetaze hemaglutininskom-oznakom i njezina detekcija na površini stanične stijenke kvasca." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:292823

Vukšić, Anđela. "Obilježavanje S-adenozil-metionin sintetaze hemaglutininskom-oznakom i njezina detekcija na površini stanične stijenke kvasca." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:292823

Vukšić, A. (2023). 'Obilježavanje S-adenozil-metionin sintetaze hemaglutininskom-oznakom i njezina detekcija na površini stanične stijenke kvasca', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 11 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:292823

Vukšić A. Obilježavanje S-adenozil-metionin sintetaze hemaglutininskom-oznakom i njezina detekcija na površini stanične stijenke kvasca [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 July 11] Available at: https://urn.nsk.hr/urn:nbn:hr:159:292823

A. Vukšić, "Obilježavanje S-adenozil-metionin sintetaze hemaglutininskom-oznakom i njezina detekcija na površini stanične stijenke kvasca", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:292823

Metadata
TitleObilježavanje S-adenozil-metionin sintetaze hemaglutininskom-oznakom i njezina detekcija na površini stanične stijenke kvasca
Title (english)HA-labeling of S-adenosyl-methionine synthetase and its detection on the surface of the yeast cell wall
AuthorAnđela Vukšić
MentorBojan Žunar (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2023-09-08, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Izlaganje proteina na staničnu stijenku kvasca inovativna je tehnika korištenja enzima u biotehnološkoj proizvodnji. Kako bi se ispitalo može li se na staničnoj stijenci kvasca Saccharomyces cerevisiae izložiti i na nju kovalentno vezati kvaščev enzim S-adenozil-metionin sintetaza (Sam1), u ovom radu konstruiran je plazmid pRSII423-ccw12sam1-3ha, koji kodira za hemaglutininskom oznakom obilježen fuzijski protein Sam1-Ccw12. Navedeni fuzijski protein, spoj enzima Sam1 i Cterminalnog dijela proteina stanične stijenke Ccw12, zatim je eksprimiran u stanicama kvasca, nakon čega je imunoblotom pokazano da on uspješno prolazi kroz sekretorni put kvasca te se nekovalentno i kovalentno, preko GPI sidra, veže na staničnu stijenku kvasca. Ovim rezultatima po prvi je puta pokazano da je enzim S-adenozil-metionin sintetazu moguće izložiti na površini stanične stijenke kvasca S. cerevisiae.
Abstract (english)
Displaying proteins on the yeast cell wall is an innovative technique for the use of enzymes in biotechnological production. To investigate the possibility of displaying and covalently attaching the yeast enzyme S-adenosylmethionine synthase (Sam1) to the cell wall of yeast Saccharomyces cerevisiae, a plasmid named pRSII423-ccw12sam1-3ha was constructed in this study. This plasmid encodes a fusion protein, Sam1-Ccw12, which is marked with a hemagglutinin tag. This fusion protein, a combination of the Sam1 enzyme and the C-terminal part of the cell wall protein Ccw12, was then expressed in yeast cells. Immunoblot analysis demonstrated that this fusion protein successfully traverses the yeast's secretory pathway and becomes non-covalently and covalently attached to the yeast cell wall via a GPI anchor. These results demonstrate, for the first time, that it is feasible to expose the enzyme S-adenosylmethionine synthase on the cell wall surface of the yeast S. cerevisiae.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:292823
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-04-30 08:52:56