prikaz prve stranice dokumenta Određivanje sastava lignoceluloznih hidrolizata pomoću tekućinske kromatografije visoke djelotvornosti
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undergraduate thesis
Određivanje sastava lignoceluloznih hidrolizata pomoću tekućinske kromatografije visoke djelotvornosti
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. urn:nbn:hr:159:463478
Mušak, Lucija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Mušak, L. (2014). Određivanje sastava lignoceluloznih hidrolizata pomoću tekućinske kromatografije visoke djelotvornosti (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:463478

Mušak, Lucija. "Određivanje sastava lignoceluloznih hidrolizata pomoću tekućinske kromatografije visoke djelotvornosti." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:463478

Mušak, Lucija. "Određivanje sastava lignoceluloznih hidrolizata pomoću tekućinske kromatografije visoke djelotvornosti." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2014. https://urn.nsk.hr/urn:nbn:hr:159:463478

Mušak, L. (2014). 'Određivanje sastava lignoceluloznih hidrolizata pomoću tekućinske kromatografije visoke djelotvornosti', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 11 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:463478

Mušak L. Određivanje sastava lignoceluloznih hidrolizata pomoću tekućinske kromatografije visoke djelotvornosti [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2014 [cited 2024 July 11] Available at: https://urn.nsk.hr/urn:nbn:hr:159:463478

L. Mušak, "Određivanje sastava lignoceluloznih hidrolizata pomoću tekućinske kromatografije visoke djelotvornosti", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2014. Available at: https://urn.nsk.hr/urn:nbn:hr:159:463478

Metadata
TitleOdređivanje sastava lignoceluloznih hidrolizata pomoću tekućinske kromatografije visoke djelotvornosti
Title (english)High-performance liquid chromatographic determination of lignocellulosic hydrolysates composition
AuthorLucija Mušak
MentorMirela Ivančić - Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2014-09-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Svjetska potražnja za fosilnim resursima za proizvodnju energije, kemikalija i materijala u stalnom je porastu, kako bi se zadovoljile potrebe brzorastućih gospodarstva zemalja poput Kine i Indije, uzrokujući kontinuirani rast cijena nafte. Proizvodnja biogoriva iz obnovljivih izvora postaje moguća alternativa fosilnim gorivima. Lignoceluloza je najzastupljeniji organski materijal na zemlji i jeftina obnovljiva sirovina za proizvodnju biogoriva. Međutim, zbog složene strukture, lignocelulozna biomasa mora biti podvrgnuta predobradi, te hidrolizi polisaharida u šećerne monomere, koje mikroorganizmi mogu asimilirati. Priprema lignocelulozne biomasa uključuje mljevenje i / ili drobljenje, te hidrolizu razrijeđenom kiselinom (obično s H2SO4). U ovom radu određene su koncentracije šećera monomera u kiselinskom hidrolizatu uobičajenih lignoceluloznih sirovina u Hrvatskoj, kukuruznog oklaska, kukuruzovine i sijena. Kiselinski hidrolizati lignoceluloze analizirani su primjenom dvije HPLC kromatografske kolone za razdvajanje šećernih monomera, Supelcogel C610H (Supelco) i Rezex RPM Pb2 + (Phenomenex). Najzastupljeniji ugljikohidrati u analiziranoj lignoceluloznoj biomasi bili su glukan i ksilan. Sijeno, kukuruzni oklasak i kukuruzovina sadržavali su 38,71%, 32,57% i 36,49% glukana, odnosno 19,99%, 29,01% i 32,01% ksilana.
Abstract (english)
Worldwide demand for fossil-resource to produce energy, chemicals and materials is steadily increasing, in order to satisfy the fast growing economies of countries such as China and India, causing continuous rise of petroleum prices. Production of biofuels from renewable resources is becoming promising alternative to fossil fuels. Lignocellulose is the most abundant organic material on earth and cheap renewable resource for biofuel production. However, due to complex structure, lignocellulosic biomass has to be subjected to pretreatment and hydrolysis of polysaccharides to sugar monomers, which can be assimilated by microorganisms. The pretreatment of lignocellulosic biomass comprises milling and/or grinding and dilute acid hydrolysis (usually with H2SO4). In this work concentrations of sugar monomers in acid hydrolysate of the common lignocellulosic biomass in Croatia, corn cob, corn husk leaves and hay, were determined. Lignocellulose acid hydrolysates were analysed by HPLC using two chromatography columns for sugar monomer separation, Supelcogel C610H (Supelco) and Rezex RPM Pb2+ (Phenomenex). Most the most abundant carbohydrates in analysed lignocellusoic biomass were glucan i xylan. Hay, corn cob and corn husk leaves contained 38,71 %, 32,57 % i 36,49 % glucan and 19,99 %, 29,01 %, 32,01 % xylan, respectively.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:463478
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2017-04-27 11:52:38