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undergraduate thesis
Primjena celulaze u ekstrakciji bioaktivnih spojeva iz lista masline
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:806512
Limari, Lora
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Limari, L. (2023). Primjena celulaze u ekstrakciji bioaktivnih spojeva iz lista masline (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:806512

Limari, Lora. "Primjena celulaze u ekstrakciji bioaktivnih spojeva iz lista masline." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:806512

Limari, Lora. "Primjena celulaze u ekstrakciji bioaktivnih spojeva iz lista masline." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:806512

Limari, L. (2023). 'Primjena celulaze u ekstrakciji bioaktivnih spojeva iz lista masline', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:806512

Limari L. Primjena celulaze u ekstrakciji bioaktivnih spojeva iz lista masline [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 August 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:806512

L. Limari, "Primjena celulaze u ekstrakciji bioaktivnih spojeva iz lista masline", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:806512

Metadata
TitlePrimjena celulaze u ekstrakciji bioaktivnih spojeva iz lista masline
Title (english)Application of cellulase in the extraction of bioactive compounds from olive leaves
AuthorLora Limari
MentorTonči Rezić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2023-07-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Enzimski potpomognute ekstrakcije su metode koje se temelje na korištenju enzima koji hidroliziraju staničnu stijenku biljaka, kako bi se povećala efikasnost ekstrakcijskog predtretmana te se smanjila količina organskog otapala koje se koristi tijekom ekstrakcije. U ovom radu određivana je aktivnosti enzima celulaze, ksilanaze i pektinaze. Određivanje je provedeno prema Kracherovoj metodi. To je kolorimetrijska metoda u kojoj DNSA uz prisutnost reducirajućih šećera prelazi u 3-amino-5-nitrosalicilnu kiselinu, te se karbonilna skupina šećera reducira u karboksilnu skupinu. U usporedbi s rezultatima određivanja aktivnosti celulaze u otopinama supstrata celuloze i lista masline, možemo zaključiti da je aktivnost veća ako je supstrat čist. Također na prinos ekstrakcije utječu i temperatura, pH, vrijeme ekstrakcije, količina enzima i vrsta enzima. Korištenjem enzima celulaze značajno se povećao prinos ekstrakcije fenola iz lista masline.
Abstract (english)
Enzyme-assisted extractions are methods based on the use of enzymes that hydrolyze plant cell walls, in order to increase the efficiency of the extraction pretreatment and reduce the amount of organic solvent used during extraction. In this thesis, cellulase, xylanase and pectinase enzyme activity was determined. The determination was carried out according to Kracher's method. It is a colorimetric method in which, in the presence of reducing sugars, DNSA turns into 3-amino-5-nitrosalicylic acid, and the carbonyl group of the sugar is reduced to a carboxyl group. In comparison with the results of the determination of cellulase activity in cellulose and olive leaf substrate solutions, we can conclude that the activity is higher if the substrate is clean. Although, extraction yield is also affected by temperature, pH, extraction time, amount of enzyme and type of enzyme. Utilization of cellulase enzyme significantly increased the yield of phenols extraction from olive leaves.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:806512
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0) icon
Created on2024-05-06 08:11:52