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undergraduate thesis
Konstrukcija plazmida pBAD-RadH
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:438504
Rohlik, Franciska
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Rohlik, F. (2023). Konstrukcija plazmida pBAD-RadH (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:438504

Rohlik, Franciska. "Konstrukcija plazmida pBAD-RadH." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:438504

Rohlik, Franciska. "Konstrukcija plazmida pBAD-RadH." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:438504

Rohlik, F. (2023). 'Konstrukcija plazmida pBAD-RadH', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 15 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:438504

Rohlik F. Konstrukcija plazmida pBAD-RadH [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 July 15] Available at: https://urn.nsk.hr/urn:nbn:hr:159:438504

F. Rohlik, "Konstrukcija plazmida pBAD-RadH", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:438504

Metadata
TitleKonstrukcija plazmida pBAD-RadH
Title (english)Construction of plasmid pBAD-RadH
AuthorFranciska Rohlik
MentorMarina Svetec Miklenić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2023-07-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Escherichia coli jedan je od najpoznatijih i najvažnijih modelnih mikroorganizama. Zbog dobrog poznavanja genetike i biologije ove bakterije, njezinog kratkog generacijskog vremena te jednostavnih uvjeta uzgoja, koristi se kao domaćin za biotehnološku proizvodnju raznih kemikalija. Razvoj velikog broj tehnika genetičkog inženjerstva bio je usmjeren upravo na ovu korisnu bakteriju, te se stoga danas njezinim genomom može relativno lako manipulirati. Cilj ovog rada je konstruirati plazmid pBAD-RadH, ekspresijski plazmid za bakteriju E. coli na kojem se pod kontrolom inducibilnog araBAD promotora nalazi gen RadH koji kodira za R-specifičnu alkohol-dehidrogenazu. Uspješnost konstrukcije plazmida pBAD-RadH potvrđena je elektroforezom i restrikcijskom analizom.
Abstract (english)
Escherichia coli is one of the best known and most important model microorganisms. Due to a good knowledge of the genetics and biology of this bacterium, its short generation time and simple growing conditions, it was used as a host for the biotechnological production of various chemicals. The development of a large number of genetic engineering techniques was aimed precisely at this useful bacterium, and therefore today its genome can be relatively easily manipulated. The aim of this work is to construct the plasmid pBAD-RadH,which is an expression plasmid for the bacterium E. coli on which, under the control of the inducible araBAD promoter, there is the RadH gene that encodes Rspecific alcohol dehydrogenase. The success of the construction of the plasmid pBAD-RadH was verified by electrophoresis and restriction analysis
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:438504
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-05-06 11:48:30