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master's thesis
Djelovanje biokonjugata ferocena i derivata 2-tiouracila na HeLa staničnu liniju
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:375783
Logarušić, Iva
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Logarušić, I. (2023). Djelovanje biokonjugata ferocena i derivata 2-tiouracila na HeLa staničnu liniju (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:375783

Logarušić, Iva. "Djelovanje biokonjugata ferocena i derivata 2-tiouracila na HeLa staničnu liniju." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:375783

Logarušić, Iva. "Djelovanje biokonjugata ferocena i derivata 2-tiouracila na HeLa staničnu liniju." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:375783

Logarušić, I. (2023). 'Djelovanje biokonjugata ferocena i derivata 2-tiouracila na HeLa staničnu liniju', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 10 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:375783

Logarušić I. Djelovanje biokonjugata ferocena i derivata 2-tiouracila na HeLa staničnu liniju [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 July 10] Available at: https://urn.nsk.hr/urn:nbn:hr:159:375783

I. Logarušić, "Djelovanje biokonjugata ferocena i derivata 2-tiouracila na HeLa staničnu liniju", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:375783

Metadata
TitleDjelovanje biokonjugata ferocena i derivata 2-tiouracila na HeLa staničnu liniju
Title (english)Activity of ferrocene bioconjugates with 2-thiouracil derivatives on HeLa cell line
AuthorIva Logarušić
MentorKristina Radošević (mentor)
MentorMartina Bagović (pomoć pri izradi, neposredni voditelj)
Committee memberSenka Djaković (predsjednik povjerenstva)
Committee memberVišnja Gaurina Srček (član povjerenstva)
Committee memberJasmina Lapić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2023-04-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Ferocenski konjugati su organometalni spojevi koji posjeduju različita elektrokemijska i biološka svojstva zbog čega se istražuju i primjenjuju kao novi biološki aktivni spojevi u procesu istraživanja i razvoja lijekova. Budući da veliki broj ferocenskih konjugata pokazuje antitumorski učinak, u ovom radu je ispitana in vitro biološka aktivnost četiri različita biokonjugata ferocena i derivata 2-tiouracila na humanoj tumorskoj staničnoj liniji HeLa određivanjem stanične vijabilnosti i klonogenom analizom. Nadalje, protočnom citometrijom određeno je s kojim se staničnim procesom može povezati zapažena citotoksičnost. Sva četiri ispitana biokonjugata pokazala su inhibitorni učinak na rast i proliferaciju HeLa stanične linije, pri čemu su spojevi 3 i 4 pokazali najizraženiji citotoksični učinak. Inhibitorno djelovanje spojeva 3 i 4 na rast HeLa stanica može se povezati s poticanjem stanične smrti procesom apoptoze. Dobiveni rezultati ukazuju da biokonjugati ferocena i derivata 2-tiouracila imaju potencijal kao antitumorski agensi.
Abstract (english)
Ferrocene conjugates are organometallic compounds that have different electrochemical and biological properties, therefore they have been investigated and used as new biologically active compounds in the process of drug research and development. A large number of ferrocene conjugates show antitumor activity, therefore in this work the in vitro biological activity of four different bioconjugates of ferrocene and 2-thiouracil derivatives was assessed on human tumor cell line HeLa by determination of cell viability and clonogenic analysis. Furthermore, flow cytometry was used to determine which cellular process could be associated with the observed cytotoxicity. All tested bioconjugates showed an inhibitory effect on the growth and proliferation of HeLa cell line and compounds 3 and 4 showed the greatest cytotoxic effect. The inhibitory effect of compounds 3 and 4 on the growth of HeLa cells can be associated with the stimulation of cell death by the process of apoptosis. The obtained results indicate that bioconjugates of ferrocene and 2-thiouracil derivatives have potential as antitumor agents.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:375783
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-05-14 11:19:46