prikaz prve stranice dokumenta Evaluation of downstream methods for monoclonal antibodies produced from CHO cell lines
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master's thesis
Evaluation of downstream methods for monoclonal antibodies produced from CHO cell lines
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:264444
Cvetković, Jakov
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Cvetković, J. (2023). Evaluation of downstream methods for monoclonal antibodies produced from CHO cell lines (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:264444

Cvetković, Jakov. "Evaluation of downstream methods for monoclonal antibodies produced from CHO cell lines." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:264444

Cvetković, Jakov. "Evaluation of downstream methods for monoclonal antibodies produced from CHO cell lines." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:264444

Cvetković, J. (2023). 'Evaluation of downstream methods for monoclonal antibodies produced from CHO cell lines', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:264444

Cvetković J. Evaluation of downstream methods for monoclonal antibodies produced from CHO cell lines [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 June 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:264444

J. Cvetković, "Evaluation of downstream methods for monoclonal antibodies produced from CHO cell lines", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:264444

Metadata
TitleEvaluation of downstream methods for monoclonal antibodies produced from CHO cell lines
Title (croatian)Evaluacija metoda za pročišćavanje monoklonalnih protutijela proizvedenih iz CHO stanične linije
AuthorJakov Cvetković
MentorIgor Slivac (mentor)
Committee memberMirela Ivančić Šantek (predsjednik povjerenstva)
Committee memberVišnja Gaurina Srček (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2023-04-04, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
CHO culture is a well-charaterized cell line that has been used for years in the field of biomanufacturing to produce various valuable pharmaceutical components, such as monoclonal antibodies that are used for the treatment of numerous diseases. Novel, more cost-effective downstream techniques and methods are continuously being developed by large companies. This thesis focused on the characterization of downstream equipment, mainly three components: depth filters (used for filtration), hollow fibers (used for concentration), and ion exchange membrane (used for anionic exchange, or AEX). For the specific process of trastuzumab purification, ErtelAlsop B4E7 filters were proven to be effective in the DNA removal step. In the critical flux experiment, the Microza hollow fibers (0.2 μm pores) could sustain the highest transmembrane flux before significant fouling occurred. Lastly, AEX was effective in removing host cell proteins/DNA.
Abstract (croatian)
CHO stanice dobro su definirana kultura koje se koriste u proizvodnji mnogih farmaceutskih komponenata, poput monoklonalnih protutijela koja se koriste za prevenciju mnogih bolesti. U svrhu smanjenja troškova, mnoga velika poduzeća kontinuirano razvijaju nove metode pročišćavanja ovih vrijednih spojeva. Tijekom ovog rada ispitana je oprema za pročišćavanje, uz naglasak na tri glavna tipa: dubinski filteri (korišteni za filtraciju), membrane (korištene za koncentraciju) i membranski adsorberi (korištene za anionsku izmjenu). Za specifičan proces pročišćavanja Trastuzumab antitijela, ErtelAlsop B4E7 filteri su dokazano efektivni. Nadalje, ispitivane Microza membrane (pore veličina 0.2 μm) su izdržale najveći protok bez pojave začepljenja. Na kraju, anionska izmjena pokazala se najefektivnijom pri uklanjanju neželjenih staničnih proteina i DNK.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:159:264444
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-05-21 13:30:50