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master's thesis
Usporedba titra virusa EDSV na supstratima pogodnim za proizvodnju cjepiva
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:905293
Uzelac, Tamara
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Uzelac, T. (2023). Usporedba titra virusa EDSV na supstratima pogodnim za proizvodnju cjepiva (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:905293

Uzelac, Tamara. "Usporedba titra virusa EDSV na supstratima pogodnim za proizvodnju cjepiva." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:905293

Uzelac, Tamara. "Usporedba titra virusa EDSV na supstratima pogodnim za proizvodnju cjepiva." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:905293

Uzelac, T. (2023). 'Usporedba titra virusa EDSV na supstratima pogodnim za proizvodnju cjepiva', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 16 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:905293

Uzelac T. Usporedba titra virusa EDSV na supstratima pogodnim za proizvodnju cjepiva [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 June 16] Available at: https://urn.nsk.hr/urn:nbn:hr:159:905293

T. Uzelac, "Usporedba titra virusa EDSV na supstratima pogodnim za proizvodnju cjepiva", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:905293

Metadata
TitleUsporedba titra virusa EDSV na supstratima pogodnim za proizvodnju cjepiva
Title (english)Comparison of EDSV virus titer on substrates suitable for vaccine production
AuthorTamara Uzelac
MentorIgor Slivac (mentor)
MentorKatarina Huić Babić (komentor)
Committee memberVišnja Gaurina Srček (predsjednik povjerenstva)
Committee memberKatarina Huić Babić (član povjerenstva)
Committee memberKsenija Durgo (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2023-11-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Virusne infekcije kod domaćih životinja predstavljaju veliki zdravstveni i ekonomski problem u svijetu. Radi prevencije širenja zaraze, brojne farmaceutske tvrtke usmjeruju svoju proizvodnju k izradi cjepiva za životinje. Jedan od čestih virusa u peradi je virus sindroma pada nesivosti (EDSV) čije su posljedice značajno smanjenje i kvalitete jaja i proizvodnje. Cilj ovog rada bio je ispitati sklonosti infekcije EDSV virusom u tri različita modela u svrhu pronalaska alternative uzgoju virusa i titracijama virusa na embrioniranim kokošjim jajima. Infektivnost virusa EDSV ispitana je na embrioniranim pačjim jajima, stanicama jetre pilećeg embrija i stanicama hepatoma pijetla titracijom inokuluma virusa na navedene supstrate. Na temelju dobivenih rezultata može se zaključiti kako su stanice jetre pilećeg embrija i stanice hepatoma pijetla podložnije infekciji virusom EDSV u odnosu na embrionirana pačja jaja te se mogu koristiti kao alternativa u istraživanjima i proizvodnji cjepiva.
Abstract (english)
Viral infections represent a major health and economic problem in the world, and in order to prevent the consequences of the spread and effects of such infections, many pharmaceutical companies have directed their production towards the production of vaccines. One of the common poultry viruses is the egg drop syndrome (EDSV), the consequences of which are a significant reduction in egg quality and production. Therefore, the aim of this study was to examine the EDSV virus infection tendencies in three different models in order to find an alternative to virus cultivation and virus titrations on embryonated chicken eggs. The infectivity of the EDSV virus was tested on embryonated duck eggs, chicken embryo liver cells and rooster hepatoma cells by titration of the virus inoculum on the mentioned substrates. According to the results, it can be concluded that chicken embryo liver cells and rooster hepatoma cells are more susceptible to infection with the EDSV virus compared to embryonated duck eggs and can be used as an alternative in research and production of vaccines.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:905293
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-05-23 09:45:03