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master's thesis
Analiza stabilnosti lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:189608
Lacić, Petra
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Lacić, P. (2023). Analiza stabilnosti lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:189608

Lacić, Petra. "Analiza stabilnosti lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:189608

Lacić, Petra. "Analiza stabilnosti lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:189608

Lacić, P. (2023). 'Analiza stabilnosti lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:189608

Lacić P. Analiza stabilnosti lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:189608

P. Lacić, "Analiza stabilnosti lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:189608

Metadata
TitleAnaliza stabilnosti lizozima u niskotemperaturnim eutektičkim otapalima na bazi osmolita
Title (english)Analysis of lysozyme stability in eutectic solvents based on osmolytes
AuthorPetra Lacić
MentorMarina Cvjetko Bubalo (mentor)
Committee memberIvana Radojčić Redovniković (predsjednik povjerenstva)
Committee memberAna Jurinjak Tušek (član povjerenstva)
Committee memberAndreja Leboš-Pavunc (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2023-11-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Proučavanjem živih sustava identificiran je specifičan mehanizam zaštite organizma u stresnim uvjetima koji se oslanja na male neutralne i netoksične molekule poznate pod imenom osmoliti. Osmoliti, osim što djeluju kao obrambeni mehanizam, imaju ključnu ulogu u održavanju termodinamičke stabilnosti biomakromolekula bez narušavanja njihove funkcionalnosti. S obzirom da se smatraju „kompleksnim adaptivnim sustavima” koje karakteriziraju emergentna svojstva, nedavno je otkrivena povezanost sa niskotemperaturnim eutektičkim otapalima koja učinkovito repliciraju prirodno okruženje različitih biomolekula, a ujedno imaju slična svojstva osmolita. U ovom radu je ispitan utjecaj niskotemperaturnih eutektičkih otapala na bazi osmolita (osmoDES) na toplinski induciranu agregaciju i termodinamičku stabilnost proteina lizozima pri ekstremnim temperaturama. S obzirom na rezultate, pripravljeni osmoDES-ovi imaju značajan potencijal za primjenu kao tekući skladišni medij proteina zbog svojih izuzetnih stabilizacijskih sposobnosti.
Abstract (english)
By studying living systems, a specific mechanism for protecting the organism in stressful conditions has been identified, which relies on small neutral and non-toxic molecules known as osmolytes. Osmolytes, apart from acting as a defense mechanism, play a crucial role in maintaining the thermodynamic stability of biomacromolecules without compromising their functionality. Considering them as „complex adaptive systems” characterized by emergent properties, a recent connection with eutectic solvents based on osmolytes (osmoDES) has been discovered, effectively replicating the natural environment of various biomolecules while sharing similar osmolyte properties. In this study, the impact of osmoDES on thermally induced aggregation and thermodynamic stability of lysozyme protein at extreme temperatures was investigated. Based on the results, the prepared osmoDES exhibit significant potential for application as a liquid storage medium for proteins due to their exceptional stabilizing capabilities.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:189608
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-05-23 10:35:53