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master's thesis
Potencijal niskotemperaturnih eutektičkih otapala za zamjenu dimetilsulfoksida
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:946689
Šarac, Sunčica
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Šarac, S. (2023). Potencijal niskotemperaturnih eutektičkih otapala za zamjenu dimetilsulfoksida (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:946689

Šarac, Sunčica. "Potencijal niskotemperaturnih eutektičkih otapala za zamjenu dimetilsulfoksida." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:946689

Šarac, Sunčica. "Potencijal niskotemperaturnih eutektičkih otapala za zamjenu dimetilsulfoksida." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:946689

Šarac, S. (2023). 'Potencijal niskotemperaturnih eutektičkih otapala za zamjenu dimetilsulfoksida', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 28 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:946689

Šarac S. Potencijal niskotemperaturnih eutektičkih otapala za zamjenu dimetilsulfoksida [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 June 28] Available at: https://urn.nsk.hr/urn:nbn:hr:159:946689

S. Šarac, "Potencijal niskotemperaturnih eutektičkih otapala za zamjenu dimetilsulfoksida", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:946689

Metadata
TitlePotencijal niskotemperaturnih eutektičkih otapala za zamjenu dimetilsulfoksida
Title (english)Potential of deep eutectic solvents to replace dimethyl sulfoxide
AuthorSunčica Šarac
MentorKristina Radošević (mentor)
MentorMartina Bagović (pomoć pri izradi, neposredni voditelj)
Committee memberAna Bielen (predsjednik povjerenstva)
Committee memberMarina Cvjetko Bubalo (član povjerenstva)
Committee memberTeuta Murati (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2023-12-22, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Zbog rastućeg broja dokaza o toksičnom djelovanju dimetilsulfoksida (DMSO) na ljudsko zdravlje potrebno je pronaći alternativna otapala koja će moći zamijeniti široko korišteni DMSO. Niskotemperaturna eutektička otapala (engl. Deep Eutectic Solvents, DES) su niskotoksična i biorazgradiva te se jednostavno pripravljaju. U ovom radu ispitana je citotoksičnost četiri DES-a (Bet:U, TMAO:Gua, TMAO:U i GPC:U) prema HeLa i HaCaT stanicama te je najslabije citotoksično djelovanje, slabije od DMSO-a, pokazao Bet:U. Odabrani DES-ovi (Bet:U, TMAO:U i ChCl:U) također su primijenjeni kao krioprotektivni agensi pri zamrzavanju HeLa i HaCaT staničnih linija, ali niti jedno niskotemperaturno eutektičko otapalo nije iskazalo krioprotektivna svojstva jednaka ili bolja DMSO-u. Primjenom COSMOtherm programa predviđena je topljivost ferocena u hidrofilnim i hidrofobnim DESovima te je pokazano da su hidrofobni DES-ovi bolji izbor za otapanje ferocena. Također je ispitana mogućnost otapanja biokonjugata ferocena i derivata 2-tiouracila u Ty:C₈ i Me:C₈ te potom učinak otopina spojeva u DES-ovima na HeLa staničnoj liniji. Pri ispitanim koncentracijama nije zapažen značajniji učinak biokonjugata ferocena i derivata 2-tiouracila na proliferaciju stanica, u odnosu na kontrolne stanice.
Abstract (english)
Increasing evidence of the toxic effects of dimethyl sulfoxide (DMSO) on human health made it necessary to find alternative solvents that can replace the widely used DMSO. Deep eutectic solvents (DES) are low-toxic, biodegradable and easily prepared. In this study, the cytotoxicity of four DESs (Bet:U, TMAO:Gua, TMAO:U, and GPC:U) towards HeLa and HaCaT cells was investigated, with Bet:U showing the least cytotoxic effect, which was lower than that of DMSO. Selected DESs (Bet:U, TMAO:U, and ChCl:U) were also applied as cryoprotective agents for HeLa and HaCaT cell lines, but none of the deep eutectic solvents showed cryoprotective properties equal to or better than DMSO. By using the COSMOtherm program, the solubility of ferrocene in hydrophilic and hydrophobic DES has been predicted, demonstrating that hydrophobic DESs are a better choice for dissolving ferrocene. Additionally, the possibility of dissolving bioconjugates of ferrocene and 2-thiouracil derivative in Ty:C₈ and Me:C₈ was examined, followed by assessing the effect of solutions on HeLa cell line. At the tested concentrations, there was no significant impact of bioconjugates of ferrocene and 2-thiouracil derivative on cell proliferation compared to control cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:946689
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-06-05 08:04:59