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master's thesis
Karakterizacija uloge proteina IqgC u adheziji stanica kod amebe Dictyostelium discoideum
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:105957
Mimica, Lucija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Mimica, L. (2023). Karakterizacija uloge proteina IqgC u adheziji stanica kod amebe Dictyostelium discoideum (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:105957

Mimica, Lucija. "Karakterizacija uloge proteina IqgC u adheziji stanica kod amebe Dictyostelium discoideum." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:105957

Mimica, Lucija. "Karakterizacija uloge proteina IqgC u adheziji stanica kod amebe Dictyostelium discoideum." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:105957

Mimica, L. (2023). 'Karakterizacija uloge proteina IqgC u adheziji stanica kod amebe Dictyostelium discoideum', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 10 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:105957

Mimica L. Karakterizacija uloge proteina IqgC u adheziji stanica kod amebe Dictyostelium discoideum [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 July 10] Available at: https://urn.nsk.hr/urn:nbn:hr:159:105957

L. Mimica, "Karakterizacija uloge proteina IqgC u adheziji stanica kod amebe Dictyostelium discoideum", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:105957

Metadata
TitleKarakterizacija uloge proteina IqgC u adheziji stanica kod amebe Dictyostelium discoideum
Title (english)A role of IqgC protein in cell adhesion in Dictyostelium discoideum amoeba
AuthorLucija Mimica
MentorIgor Slivac (mentor)
MentorIgor Weber (komentor)
MentorLucija Mijanović (pomoć pri izradi, neposredni voditelj)
Committee memberKristina Radošević (predsjednik povjerenstva)
Committee memberIgor Weber (član povjerenstva)
Committee memberIgor Stuparević (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2023-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Protein IqgC sličan je proteinima IQGAP, a ima svojstva i RasGAP proteina. Prethodno istraživanje je pokazalo da protein IqgC regulira fagocitozu i makropinocitozu deaktivacijom male GTPaze RasG. Cilj ovog rada bio je otkriti ima li protein IqgC ulogu u staničnoj adheziji i ostvaruje li interakciju s malom GTPazom RapA, koja ima ulogu u regulaciji stanične adhezije. Metodama pull-down i koimunoprecipitacije potvrđena je interakcija proteina IqgC s proteinom RapA divljeg tipa, konstitutivno negativnim mutantom S19N i konstitutivno aktivnom formom Q65E. Testom pojedinačnih stanica, u kojem su korištene stanice deficijentne za protein IqgC (iqgC- ), utvrđeno je da protein IqgC sudjeluje u adheziji osjetljivoj na EDTA.
Abstract (english)
IqgC protein shares properties of both IQGAP and RasGAP proteins. It is already known that IqgC is involved in the regulation of macropinocytosis and phagocytosis by deactivating a small GTPase protein RasG . The goals of this paper were to investigate the potential role of IqgC in the regulation of cell adhesion and interaction with the small GTPase RapA. Interaction of the IqgC with wild type, constitutively inactive mutant (S19N) and constitutively active form (Q65E) of RapA was confirmed by using pull-down assay and coimmunoprecipitation assays. The results of the single-cell adhesion assay also implicate IqgC in the regulation of the EDTA-sensitive cell adhesion.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:105957
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-06-06 10:25:01