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master's thesis
Povezanost antilisterijske i antistafilokokne aktivnosti Lactiplantibacillus plantarum sojeva i fenotipa sinteze plantaricina
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:076685
Perica, Jana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Perica, J. (2023). Povezanost antilisterijske i antistafilokokne aktivnosti Lactiplantibacillus plantarum sojeva i fenotipa sinteze plantaricina (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:076685

Perica, Jana. "Povezanost antilisterijske i antistafilokokne aktivnosti Lactiplantibacillus plantarum sojeva i fenotipa sinteze plantaricina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:076685

Perica, Jana. "Povezanost antilisterijske i antistafilokokne aktivnosti Lactiplantibacillus plantarum sojeva i fenotipa sinteze plantaricina." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:076685

Perica, J. (2023). 'Povezanost antilisterijske i antistafilokokne aktivnosti Lactiplantibacillus plantarum sojeva i fenotipa sinteze plantaricina', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 24 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:076685

Perica J. Povezanost antilisterijske i antistafilokokne aktivnosti Lactiplantibacillus plantarum sojeva i fenotipa sinteze plantaricina [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 June 24] Available at: https://urn.nsk.hr/urn:nbn:hr:159:076685

J. Perica, "Povezanost antilisterijske i antistafilokokne aktivnosti Lactiplantibacillus plantarum sojeva i fenotipa sinteze plantaricina", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:076685

Metadata
TitlePovezanost antilisterijske i antistafilokokne aktivnosti Lactiplantibacillus plantarum sojeva i fenotipa sinteze plantaricina
Title (english)Association of antilisteric and antistaphylococcal activity of Lactiplantibacillus plantarum strains and plantaricin synthesis phenotype
AuthorJana Perica
MentorJasna Novak (mentor)
MentorKatarina Butorac (pomoć pri izradi, neposredni voditelj)
MentorNina Čuljak (pomoć pri izradi, neposredni voditelj)
Committee memberBlaženka Kos (predsjednik povjerenstva)
Committee memberKsenija Markov (član povjerenstva)
Committee memberAnita Slavica (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2023-07-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Pojedini Lactiplantibacillus plantarum sojevi sintetiziraju antimikrobne metabolite bakteriocine, čiji aplikativni aspekt obuhvaća primjenu u biokonzerviranju, ali je i potencijalna strategija za smanjenje antibiotičke rezistencije. Cilj ovog diplomskog rada bio je odrediti povezanost fenotipa sinteze bakteriocina s antimikrobnim učinkom L. plantarum sojeva prema Listeria monocytogenes ATCC®19111™ i Staphylococcus aureus 3048. Supernatant kulture L. plantarum sojeva je inhibirao formiranja biofilmova test-mikroorganizama. PCR metodom potvrđena je prisutnost gena plnA, plnEF i plnJ u genomu L. plantarum sojeva. Tijekom kultivacije Lactiplantibacillus sojeva uz određivanje antimikrobne aktivnosti, praćen je bakterijski rast i acidifikacija. Prema rezultatima, sojevi L. plantarum SF15C i L. plantarum D13 iskazuju značajno inhibicijsko djelovanje tijekom stacionarne faze rasta, koje se pripisuje proizvedenim plantaricinima. Gubitak antimikrobnog djelovanja supernatanata kulture pojedinih Lactiplantibacillus sojeva pripisuje se inaktivaciji bakteriocinske aktivnosti. Ovi rezultati su temelj za daljnju izolaciju i karakterizaciju plantaricina koje proizvode L. plantarum D13, s ciljem primjene u fermentacijskim procesima za smanjenje kontaminacija ovim patogenima.
Abstract (english)
Certain Lactiplantibacillus plantarum strains synthesize antimicrobial metabolites bacteriocins, with applications in biopreservation and potential as an alternative antimicrobial strategy. This thesis aimed to examine the correlation between bacteriocin synthesis phenotype and the antimicrobial effect of L. plantarum strains against Listeria monocytogenes ATCC®19111™ and Staphylococcus aureus 3048. The culture supernatant of L. plantarum strains inhibited biofilm formation by the test-microorganisms. PCR analysis confirmed the presence of plnA, plnEF, and plnJ genes in the L. plantarum genome. During cultivation of Lactiplantibacillus strains, bacterial growth, acidification, and antimicrobial activity were monitored. L.plantarum SF15C and D13 strains exhibited a significant inhibitory effect during the stationary growth phase, attributed to plantaricin production. The reduction in antimicrobial activity in the culture supernatants of certain Lactiplantibacillus strains is attributed to bacteriocin inactivation. These findings lay the groundwork for further isolation and characterization of plantaricins produced by L. plantarum D13, facilitating their application in fermentation processes to mitigate contamination.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:076685
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-06-07 07:22:38