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master's thesis
Identifikacija i kvantifikacija bioaktivnih peptida proizvedenih s pomoću soja Lactococcus lactis ZGBP5-51
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:495075
Rušanac, Anamarija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Rušanac, A. (2023). Identifikacija i kvantifikacija bioaktivnih peptida proizvedenih s pomoću soja Lactococcus lactis ZGBP5-51 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:495075

Rušanac, Anamarija. "Identifikacija i kvantifikacija bioaktivnih peptida proizvedenih s pomoću soja Lactococcus lactis ZGBP5-51." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:495075

Rušanac, Anamarija. "Identifikacija i kvantifikacija bioaktivnih peptida proizvedenih s pomoću soja Lactococcus lactis ZGBP5-51." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:495075

Rušanac, A. (2023). 'Identifikacija i kvantifikacija bioaktivnih peptida proizvedenih s pomoću soja Lactococcus lactis ZGBP5-51', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 10 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:495075

Rušanac A. Identifikacija i kvantifikacija bioaktivnih peptida proizvedenih s pomoću soja Lactococcus lactis ZGBP5-51 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 July 10] Available at: https://urn.nsk.hr/urn:nbn:hr:159:495075

A. Rušanac, "Identifikacija i kvantifikacija bioaktivnih peptida proizvedenih s pomoću soja Lactococcus lactis ZGBP5-51", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:495075

Metadata
TitleIdentifikacija i kvantifikacija bioaktivnih peptida proizvedenih s pomoću soja Lactococcus lactis ZGBP5-51
Title (english)Identification and quantification of bioactive peptides produced by Lactococcus lactis ZGBP5-51
AuthorAnamarija Rušanac
MentorBlaženka Kos (mentor)
MentorAna Butorac (komentor)
MentorKatarina Butorac (pomoć pri izradi, neposredni voditelj)
Committee memberAndreja Leboš-Pavunc (predsjednik povjerenstva)
Committee memberAna Butorac (član povjerenstva)
Committee memberAnita Slavica (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2023-07-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovog diplomskog rada bio je identificirati i kvantificirati bioaktivne peptide nastale u uzorcima mlijeka tijekom fermentacije proteolitičkom aktivnošću radnog soja Lactococcus lactis ZGBP5-51. Identifikacija bioaktivnih peptida provedena je spregnutom tehnikom tekućinske kromatografije i spektrometrije masa LC-MALDI-TOF/TOF, a relativna kvantifikacija identificiranih bioaktivnih peptida spregnutom tehnikom tekućinske kromatografije, višestrukog praćenja reakcije i spektrometrije masa LC-MRM-MS. Ne-ciljanom analizom identificirani su bioaktivni peptidi TKVIPYVRYL, VYPFPGPIPN, ARHPHPHLSFM, KYIPIQYVL, QEPVLGPVRGPFPIIV, IQPKTKVIPYVRYL i YQEPVLGPVRGPFPIIV, dok su ciljanom analizom identificirana još tri bioaktivna peptida: RDMPIQAF, SQSKVLPVPQKAVPYPQ i LYQEPVLGPVRGPFPIIV. U znanstvenoj literaturi za navedene peptide utvrđeno je i opisano ACE-inhibitorno (antihipertenzivno), antitrombotično, antioksidativno, antimikrobno i imunomodulatorno djelovanje. Ciljanom analizom uspješno je kvantificirano osam prethodno identificiranih bioaktivnih peptida. Konačno, statistički značajan porast koncentracije, nakon 48 sati fermentacije, dokazan je za šest peptida: RDMPIQAF, VYPFPGPIPN, QEPVLGPVRGPFPIIV, SQSKVLPVPQKAVPYPQ, YQEPVLGPVRGPFPIIV i ARHPHPHLSFM.
Abstract (english)
The aim of this thesis was to identify and quantify bioactive peptides produced during milk fermentation by proteolitic activity of the strain Lactococcus lactis ZGBP5-51. Identification of bioactive peptides was performed using the liquid chromatography and mass spectrometry LC-MALDI TOF/TOF technique, while relative quantification of identified bioactive peptides was performed using the liquid chromatography, multiple reaction monitoring and mass spectrometry LC-MRM-MS technique. The non-targeted analysis identified bioactive peptides TKVIPYVRYL, VYPFPGPIPN, ARHPHPHLSFM, KYIPIQYVL, QEPVLGPVRGPFPIIV, IQPKTKVIPYVRYL, and YQEPVLGPVRGPFPIIV, while the targeted analysis identified three additional bioactive peptides: RDMPIQAF, SQSKVLPVPQKAVPYPQ, and LYQEPVLGPVRGPFPIIV. ACE-inhibitory (antihypertensive), antithrombotic, antioxidant, antimicrobial, and immunomodulatory effects have been determined and described in the scientific literature for the aforementioned peptides. Through targeted analyses, eight previously identified bioactive peptides were successfully quantified. Finally, statistically significant increase in concentration, after 48 hours of fermentation, was detected for six previously identified bioactive peptides: RDMPIQAF, VYPFPGPIPN, QEPVLGPVRGPFPIIV, SQSKVLPVPQKAVPYPQ, YQEPVLGPVRGPFPIIV and ARHPHPHLSFM.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:495075
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-06-11 08:51:26