Utjecaj utišavanja klatrina na prijenos signala MAP kinaznog puta i prepoznavanje infekcije pomoću proteina MyD88 i IFI16 nakon infekcije adenovirusom tipa 26

Lač, Maša

prikaz prve stranice dokumenta Utjecaj utišavanja klatrina na prijenos signala MAP kinaznog puta i prepoznavanje infekcije pomoću proteina MyD88 i IFI16 nakon infekcije adenovirusom tipa 26

master's thesis

Utjecaj utišavanja klatrina na prijenos signala MAP kinaznog puta i prepoznavanje infekcije pomoću proteina MyD88 i IFI16 nakon infekcije adenovirusom tipa 26

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:900941

Lač, Maša

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

APA 6th Edition

Lač, M. (2023). Utjecaj utišavanja klatrina na prijenos signala MAP kinaznog puta i prepoznavanje infekcije pomoću proteina MyD88 i IFI16 nakon infekcije adenovirusom tipa 26 (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:900941

MLA 8th Edition

Lač, Maša. "Utjecaj utišavanja klatrina na prijenos signala MAP kinaznog puta i prepoznavanje infekcije pomoću proteina MyD88 i IFI16 nakon infekcije adenovirusom tipa 26." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:900941

Chicago 17th Edition

Harvard

Lač, M. (2023). 'Utjecaj utišavanja klatrina na prijenos signala MAP kinaznog puta i prepoznavanje infekcije pomoću proteina MyD88 i IFI16 nakon infekcije adenovirusom tipa 26', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:900941

Vancouver

Lač M. Utjecaj utišavanja klatrina na prijenos signala MAP kinaznog puta i prepoznavanje infekcije pomoću proteina MyD88 i IFI16 nakon infekcije adenovirusom tipa 26 [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2025 January 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:900941

IEEE

M. Lač, "Utjecaj utišavanja klatrina na prijenos signala MAP kinaznog puta i prepoznavanje infekcije pomoću proteina MyD88 i IFI16 nakon infekcije adenovirusom tipa 26", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:900941

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:900941

Metadata

Title	Utjecaj utišavanja klatrina na prijenos signala MAP kinaznog puta i prepoznavanje infekcije pomoću proteina MyD88 i IFI16 nakon infekcije adenovirusom tipa 26
Title (english)	Effect of clathrin knockdown on MAP kinase signaling pathway and recognition of infection by proteins MyD88 and IFI16 after adenovirus type 26 infection
Author	Maša Lač
Mentor	Ksenija Durgo (mentor)
Mentor	Dragomira Majhen (komentor)
Mentor	Isabela Drašković (pomoć pri izradi, neposredni voditelj)
Committee member	Marina Cvjetko Bubalo (predsjednik povjerenstva)
Committee member	Dragomira Majhen (član povjerenstva)
Committee member	Ivana Radojčić Redovniković (član povjerenstva)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Biochemical Engineering) (Laboratory for Biology and Microbial Genetics) Zagreb
Defense date and country	2023-07-14, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Abstract	Adenovirusni vektori se koriste u genskoj terapiji, terapiji tumora i razvoju cjepiva. Zbog visoke seroprevalencije najčešće korištenih adenovirusnih vektora, sve je više istraživanja usmjereno prema drugim adenovirusima, poput adenovirusa tipa 26 (HAdV26), koji je zbog niske seroprevalencije kandidat s visokim potencijalom za primjenu kao vektorsko cjepivo. U ovom je radu ispitana uloga klatrina u prijenosu signala MAP kinaznog puta nakon infekcije HAdV26 praćenjem aktivacije kinaza ERK i p38, kao i u prepoznavanju infekcije pomoću proteina MyD88 i IFI16. Nije zabilježena aktivacija kinaze ERK nakon infekcije HAdV26, dok je aktivacija p38 uočena u staničnoj liniji s najmanjom ekspresijom klatrina. Nakon infekcije HAdV26 uočen je unutarstanični transport proteina MyD88 i IFI16, što upućuje na njihovu uključenost u prepoznavanju infekcije HAdV26.
Abstract (english)	Adenoviral vectors are used in gene therapy, cancer therapy and vaccine development. Due to high seroprevalence of the most frequently used adenovirus vectors, recent research is directed towards other adenoviruses, such as adenovirus type 26 (HAdV26), which is a candidate with great potential for vaccine vector due to its low seroprevalence. In this work, the role of clathrin in MAP kinase pathway signalling after HAdV26 infection was examined by monitoring activation of ERK and p38 kinases, as well as in the recognition of infection by MyD88 and IFI16 proteins. No activation of ERK kinase was detected after HAdV26 infection, while p38 activation was observed in the cell line with the lowest clathrin expression. After HAdV26 infection, intracellular transport of proteins MyD88 and IFI16 was observed, indicating their role in recognition of HAdV26 infection.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:900941
Study programme	Title: Molecular Biotechnology Study programme type: university Study level: graduate Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Created on	2024-06-12 09:06:52

Search form