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master's thesis
Analiza rezultata sekvenciranja i povezanost konzistencije i učestalosti stolice sa sastavom crijevne mikrobiote
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:137052
Eranović, Ivana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Section for Bioinformatics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Eranović, I. (2023). Analiza rezultata sekvenciranja i povezanost konzistencije i učestalosti stolice sa sastavom crijevne mikrobiote (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:137052

Eranović, Ivana. "Analiza rezultata sekvenciranja i povezanost konzistencije i učestalosti stolice sa sastavom crijevne mikrobiote." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:137052

Eranović, Ivana. "Analiza rezultata sekvenciranja i povezanost konzistencije i učestalosti stolice sa sastavom crijevne mikrobiote." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:137052

Eranović, I. (2023). 'Analiza rezultata sekvenciranja i povezanost konzistencije i učestalosti stolice sa sastavom crijevne mikrobiote', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 05 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:137052

Eranović I. Analiza rezultata sekvenciranja i povezanost konzistencije i učestalosti stolice sa sastavom crijevne mikrobiote [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 November 05] Available at: https://urn.nsk.hr/urn:nbn:hr:159:137052

I. Eranović, "Analiza rezultata sekvenciranja i povezanost konzistencije i učestalosti stolice sa sastavom crijevne mikrobiote", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:137052

Metadata
TitleAnaliza rezultata sekvenciranja i povezanost konzistencije i učestalosti stolice sa sastavom crijevne mikrobiote
Title (english)Analysis of sequencing results and association between stool consistency and frequency and gut microbiota composition
AuthorIvana Eranović
MentorJurica Žučko (mentor)
Committee memberIvana Rumora Samarin (predsjednik povjerenstva)
Committee memberAndreja Leboš-Pavunc (član povjerenstva)
Committee memberIgor Slivac (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Section for Bioinformatics)
Zagreb
Defense date and country2023-07-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Crijevna mikrobiota vrlo je dinamičan sustav, a na njegov sastav značajno utječu različiti obrasci prehrane, ali i mnogi drugi čimbenici. Zanemarivanje utjecaja pojedinih čimbenika na sastav mikrobiote može značajno utjecati na interpretaciju rezultata dobivenih istraživanjima. Učestalost i konzistencija stolice su mjere intestinalnog tranzitnog vremena – čimbenika koji determinira sadržaj vode i nutrijenata u crijevu te time oblikuje uvjete mikrobnog staništa. Cilj ovoga rada bio je povezati učestalost i konzistenciju stolice sa sastavom crijevne mikrobiote. Istraživanje je provedeno na 59 ispitanika od kojih su prikupljeni uzorci stolice iz kojih je određivan sastav mikrobiote sekvenciranjem 16S rRNA gena na Illumina MiSeq uređaju, a informacije o učestalosti i konzistenciji stolice dobiveni su ispunjavanjem upitnika. Za analizu podataka dobivenih sekvenciranjem korišten je program QIIME2 te DADA2 metoda pročišćavanja podataka. Prema rezultatima ove analize nije uočena značajna povezanost sastava crijevne mikrobiote s učestalosti i/ili konzistencijom stolice na promatranoj skupini ispitanika.
Abstract (english)
Gut microbiota is a very dynamic system, and its composition is strongly affected by individual’s diet pattern, as well as numerous other factors. Disregarding the impact of these factors on microbiota composition can significantly affect the interpretation of results obtained in the study. Stool consistency and frequency are used as proxies for intestinal transit time – a factor determining water and nutrient content in the intestine and, consequently, shaping the conditions of the microbial habitat. The aim of this study was to observe connection between stool consistency and frequency and gut microbiota composition. Study was conducted on 59 subjects from whom stool samples were collected and analyzed by sequencing 16S rRNA gene on Illumina MiSeq platform to determine microbiota composition. Data considering stool consistency and frequency was collected via online questionnaire. QIIME2 pipeline was used for analysis of sequencing data including DADA2 method for denoising step. The results of this study showed no significant association between stool consistency and/or frequency and gut microbiota composition in the observed group of subjects.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:137052
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NonCommercial-NoDerivs 3.0 Unported (CC BY-NC-ND 3.0) icon
Created on2024-06-14 11:29:24