prikaz prve stranice dokumenta Analiza transformanata kvasca Scheffersomyces stipitis metodom hibridizacije DNA po Southernu
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undergraduate thesis
Analiza transformanata kvasca Scheffersomyces stipitis metodom hibridizacije DNA po Southernu
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. urn:nbn:hr:159:483065
Merdžo, Martina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Merdžo, M. (2023). Analiza transformanata kvasca Scheffersomyces stipitis metodom hibridizacije DNA po Southernu (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:483065

Merdžo, Martina. "Analiza transformanata kvasca Scheffersomyces stipitis metodom hibridizacije DNA po Southernu." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:483065

Merdžo, Martina. "Analiza transformanata kvasca Scheffersomyces stipitis metodom hibridizacije DNA po Southernu." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2023. https://urn.nsk.hr/urn:nbn:hr:159:483065

Merdžo, M. (2023). 'Analiza transformanata kvasca Scheffersomyces stipitis metodom hibridizacije DNA po Southernu', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 15 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:483065

Merdžo M. Analiza transformanata kvasca Scheffersomyces stipitis metodom hibridizacije DNA po Southernu [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2023 [cited 2024 July 15] Available at: https://urn.nsk.hr/urn:nbn:hr:159:483065

M. Merdžo, "Analiza transformanata kvasca Scheffersomyces stipitis metodom hibridizacije DNA po Southernu", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:159:483065

Metadata
TitleAnaliza transformanata kvasca Scheffersomyces stipitis metodom hibridizacije DNA po Southernu
Title (english)Analysis of the Scheffersomyces stipitis transformants by Southern blot hybridization
AuthorMartina Merdžo
MentorIvan-Krešimir Svetec (mentor)
MentorMarina Svetec Miklenić (pomoć pri izradi, neposredni voditelj)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2023-07-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovog rada bio je analiza nekonvencionalnog kvasca Scheffersomyces stipitis, prethodno transformiranog s dvije različite linearne molekule DNA, kako bi se dokazala ilegitimna integracija ovih molekula u genom kvasca. Svaki transformirajući fragment nositelj je po jednog od dva gena potrebna za pretvorbu piruvata u vrijedni 2,3-butandiol te gena za rezistenciju na antibiotik, koji je korišten kao genetički biljeg za selekciju transformanata. Molekularna analiza provedena je metodom hibridizacije DNA po Southernu. Probe obilježene digoksigeninom za detekciju integriranih fragmenata sintetizirane su metodom PCR (eng. Polymerase Chain Reaction, lančana reakcija polimerazom). Probe su konstruirane tako da budu komplementarne samo kodirajućoj DNA (ORF, eng. Open Reading Frame, otvoreni okvir čitanja) gena za odgovarajuću antibiotičku rezistenciju na transformirajućim fragmentima. Analiza je pokazala da je u svim transformantima uspješno provedena integracija obje transformirajuće molekule DNA na nasumičnim mjestima u genomu kvasca.
Abstract (english)
The aim of this work was to analyse the non-conventional yeast Scheffersomyces stipitis, previously transformed with two different linear DNA molecules, to prove the illegitimate integration of these molecules into the yeast's genome. Each transforming DNA fragment contains one of two genes required for the conversion of pyruvate to a valuable 2,3-butanediol and an antibiotic resistance gene as a genetic marker for the selection of transformants. The molecular analysis was conducted by Southern blot hybridization. Digoxigenin labelled probes for detection of integrated fragments were synthesized by polymerase chain reaction (PCR). The probes were designed to be complementary only to coding DNA (Open Reading Frame, ORF) of antibiotic resistance genes on the transforming fragments. The analysis showed that the integration of two transforming DNA molecules at a random place in this yeast's genome was successful in all of the analysed transformants.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:483065
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2024-06-17 12:06:58